Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3505 | 5' | -61 | NC_001623.1 | + | 16405 | 0.66 | 0.687385 |
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Target: 5'- ---aGCGUGCCcaaGCCGGUGuACaGcGUGCCc -3' miRNA: 3'- cccaUGCACGG---CGGCCGC-UG-C-CACGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 68264 | 0.67 | 0.638492 |
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Target: 5'- ----cCGUGCCGCCGuGCGACaacaagucUGCCg -3' miRNA: 3'- cccauGCACGGCGGC-CGCUGcc------ACGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 45971 | 0.67 | 0.638492 |
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Target: 5'- cGGGUAaaGUGCUGCgaucuuaauuaCGGCGGCGucccGCCg -3' miRNA: 3'- -CCCAUg-CACGGCG-----------GCCGCUGCca--CGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 108472 | 0.67 | 0.62867 |
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Target: 5'- uGGUGUGUGCCGgCGGau-UGGUGCa -3' miRNA: 3'- cCCAUGCACGGCgGCCgcuGCCACGg -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 6337 | 0.68 | 0.609041 |
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Target: 5'- aGGcACGggagaaGgUGgUGGCGGCGGUGCCg -3' miRNA: 3'- cCCaUGCa-----CgGCgGCCGCUGCCACGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 51961 | 0.68 | 0.566151 |
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Target: 5'- aGGGU-UGUGCCGCacacacuuuGCGGUGCCg -3' miRNA: 3'- -CCCAuGCACGGCGgccgc----UGCCACGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 55794 | 0.69 | 0.531646 |
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Target: 5'- ---gGCG-GCUGUguUGGCGGCGGUGUCg -3' miRNA: 3'- cccaUGCaCGGCG--GCCGCUGCCACGG- -5' |
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| 3505 | 5' | -61 | NC_001623.1 | + | 66796 | 1.13 | 0.000592 |
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Target: 5'- cGGGUACGUGCCGCCGGCGACGGUGCCu -3' miRNA: 3'- -CCCAUGCACGGCGGCCGCUGCCACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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