Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3506 | 3' | -53.2 | NC_001623.1 | + | 52247 | 0.66 | 0.951362 |
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Target: 5'- -uUGCUGCgAUucacGCGcCggCAGUGCGCu -3' miRNA: 3'- uuGCGACGgUAu---CGCaGa-GUCAUGCG- -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 92457 | 0.66 | 0.946981 |
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Target: 5'- uAACGCguacaugacaaUGUUGUGGCGaaUgUCGGUGCGCu -3' miRNA: 3'- -UUGCG-----------ACGGUAUCGC--AgAGUCAUGCG- -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 102184 | 0.66 | 0.942346 |
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Target: 5'- uAACGCUGCaggcGCGUCUCc--GCGUg -3' miRNA: 3'- -UUGCGACGguauCGCAGAGucaUGCG- -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 91193 | 0.68 | 0.896014 |
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Target: 5'- --aGCUGCCAuaguUAGCGgcgCUCGGUAa-- -3' miRNA: 3'- uugCGACGGU----AUCGCa--GAGUCAUgcg -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 79435 | 0.72 | 0.687512 |
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Target: 5'- aGGCGCcgGCCAcAGCGUCguuGUACGUu -3' miRNA: 3'- -UUGCGa-CGGUaUCGCAGaguCAUGCG- -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 23273 | 0.73 | 0.613201 |
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Target: 5'- -uCGCUGCCA-GGUGUCUUcauuAGUAUGCc -3' miRNA: 3'- uuGCGACGGUaUCGCAGAG----UCAUGCG- -5' |
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| 3506 | 3' | -53.2 | NC_001623.1 | + | 87858 | 1.1 | 0.003166 |
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Target: 5'- cAACGCUGCCAUAGCGUCUCAGUACGCg -3' miRNA: 3'- -UUGCGACGGUAUCGCAGAGUCAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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