Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3506 | 5' | -62.5 | NC_001623.1 | + | 65327 | 0.66 | 0.637663 |
Target: 5'- cAUGCGGGCGgcgguguuguaGUCGUaCGCGCUcGCGc -3' miRNA: 3'- -UGCGCCUGCag---------CAGCG-GCGCGGuCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 54289 | 0.66 | 0.629838 |
Target: 5'- -aGCuuuGuCGUCGgCGCCGCaGCCGGCa -3' miRNA: 3'- ugCGc--CuGCAGCaGCGGCG-CGGUCGc -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 20377 | 0.66 | 0.629838 |
Target: 5'- uUGCGaGucuuCGUCGggCGCCGCGCUguuGGUGu -3' miRNA: 3'- uGCGC-Cu---GCAGCa-GCGGCGCGG---UCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 65904 | 0.66 | 0.620057 |
Target: 5'- cACGUaGugGUaguaaUCGCCGcCGCCGGCa -3' miRNA: 3'- -UGCGcCugCAgc---AGCGGC-GCGGUCGc -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 95046 | 0.66 | 0.610285 |
Target: 5'- aGCGCaauuGGAUGgcaCGUCGCCcuucauaaGCCGGCGc -3' miRNA: 3'- -UGCG----CCUGCa--GCAGCGGcg------CGGUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 23310 | 0.67 | 0.552183 |
Target: 5'- aACGC-GACGcCGUgcuuaUGCCGUGUCGGCa -3' miRNA: 3'- -UGCGcCUGCaGCA-----GCGGCGCGGUCGc -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 39802 | 0.67 | 0.552183 |
Target: 5'- gACGUGGGCGU-GUUGgCGUGCUuuuuAGCGc -3' miRNA: 3'- -UGCGCCUGCAgCAGCgGCGCGG----UCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 105680 | 0.67 | 0.541691 |
Target: 5'- uUGUGGGCGUCGcCauguacgGCCGCGgcuggaCCGGCGu -3' miRNA: 3'- uGCGCCUGCAGCaG-------CGGCGC------GGUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 101390 | 0.67 | 0.533157 |
Target: 5'- cCGCGGAaaaaaacucuCGUCGUacauuaCGUCGCGCaCGGUGc -3' miRNA: 3'- uGCGCCU----------GCAGCA------GCGGCGCG-GUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 66617 | 0.68 | 0.505077 |
Target: 5'- cGCGCGuGGCGaCgGUCGuaggcaCCGuCGCCGGCGg -3' miRNA: 3'- -UGCGC-CUGCaG-CAGC------GGC-GCGGUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 87759 | 0.68 | 0.505077 |
Target: 5'- uGCGCGaGCcaaCGcCGCCGCGCCcGCGc -3' miRNA: 3'- -UGCGCcUGca-GCaGCGGCGCGGuCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 33775 | 0.68 | 0.477653 |
Target: 5'- aACGCGGGCccacguGUUGgCGuuGCGCCAGa- -3' miRNA: 3'- -UGCGCCUG------CAGCaGCggCGCGGUCgc -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 12097 | 0.69 | 0.41674 |
Target: 5'- -gGUGGACGggGU-GCCGCGCCAcgGCGc -3' miRNA: 3'- ugCGCCUGCagCAgCGGCGCGGU--CGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 127262 | 0.7 | 0.392124 |
Target: 5'- gACGCucgGGACGgCGUCGaaGCGCUGGUGu -3' miRNA: 3'- -UGCG---CCUGCaGCAGCggCGCGGUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 55692 | 0.71 | 0.345822 |
Target: 5'- -gGCGGugGUUGcaUCGCgggcacggGCGCCGGCGg -3' miRNA: 3'- ugCGCCugCAGC--AGCGg-------CGCGGUCGC- -5' |
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3506 | 5' | -62.5 | NC_001623.1 | + | 87824 | 1.07 | 0.001041 |
Target: 5'- aACGCGGACGUCGUCGCCGCGCCAGCGu -3' miRNA: 3'- -UGCGCCUGCAGCAGCGGCGCGGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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