Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3507 | 3' | -52.5 | NC_001623.1 | + | 65498 | 0.66 | 0.972588 |
Target: 5'- -aG-CGCGAGCGCGuacGAcuacaacACCGCCGc -3' miRNA: 3'- uaCaGCGUUCGUGCuu-CUu------UGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 14612 | 0.66 | 0.972588 |
Target: 5'- cAUGUCGguGugaucguugacGCACGAGuuguacGAuuCCGCCGg -3' miRNA: 3'- -UACAGCguU-----------CGUGCUU------CUuuGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 6317 | 0.66 | 0.967743 |
Target: 5'- gGUGgaggCGCuggaauguuAGGCACgGGAGAAggugguggcggcgguGCCGCCGg -3' miRNA: 3'- -UACa---GCG---------UUCGUG-CUUCUU---------------UGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 34280 | 0.66 | 0.966442 |
Target: 5'- -cGUCgGCAAGCACGccGccguuGGGCCgGCCGu -3' miRNA: 3'- uaCAG-CGUUCGUGCuuC-----UUUGG-CGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 55680 | 0.66 | 0.963029 |
Target: 5'- --aUCGCGGGCACGGGc--GCCGgCGg -3' miRNA: 3'- uacAGCGUUCGUGCUUcuuUGGCgGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 66183 | 0.66 | 0.963029 |
Target: 5'- cUGUUGCucAAGCACGccacucuGCCGCCa -3' miRNA: 3'- uACAGCG--UUCGUGCuucuu--UGGCGGc -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 102007 | 0.67 | 0.955495 |
Target: 5'- -aGUCGCGcGuCACGAuauccacgcGGAGACgCGCCu -3' miRNA: 3'- uaCAGCGUuC-GUGCU---------UCUUUG-GCGGc -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 129489 | 0.67 | 0.955495 |
Target: 5'- aGUGUgGC-GGCGCuaaacAAGAAauaGCCGCCGg -3' miRNA: 3'- -UACAgCGuUCGUGc----UUCUU---UGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 28087 | 0.67 | 0.951362 |
Target: 5'- uUGU-GUgcGCACgGAAGGAGCUGCCa -3' miRNA: 3'- uACAgCGuuCGUG-CUUCUUUGGCGGc -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 30935 | 0.67 | 0.951362 |
Target: 5'- cGUG-CGCGAGUuugugGAAGAAACUgGCCGg -3' miRNA: 3'- -UACaGCGUUCGug---CUUCUUUGG-CGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 119893 | 0.67 | 0.951362 |
Target: 5'- cGUG-CGCAuGUugGGAGAGACUuacaaaGCCGc -3' miRNA: 3'- -UACaGCGUuCGugCUUCUUUGG------CGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 129575 | 0.67 | 0.946981 |
Target: 5'- uAUGU-GCAAgguaaagacGC-CGGAGAAAUCGCCGa -3' miRNA: 3'- -UACAgCGUU---------CGuGCUUCUUUGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 129387 | 0.68 | 0.926904 |
Target: 5'- uUGUCGC-GGCAgCGAuccguCCGCCGa -3' miRNA: 3'- uACAGCGuUCGU-GCUucuuuGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 61849 | 0.7 | 0.850825 |
Target: 5'- cGUGUCGCAucuaAGCgACGAAGAcgacgcgacgguGAUCGCUa -3' miRNA: 3'- -UACAGCGU----UCG-UGCUUCU------------UUGGCGGc -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 75630 | 0.71 | 0.806313 |
Target: 5'- gGUGUUGCAGGCGaagcaggUGGAGGAAuaGCCGu -3' miRNA: 3'- -UACAGCGUUCGU-------GCUUCUUUggCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 31054 | 0.71 | 0.797963 |
Target: 5'- -cGUCGCGcguaccggAGCGCGAAcgcgcccGCCGCCGa -3' miRNA: 3'- uaCAGCGU--------UCGUGCUUcuu----UGGCGGC- -5' |
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3507 | 3' | -52.5 | NC_001623.1 | + | 88395 | 1.06 | 0.007314 |
Target: 5'- cAUGUCGCAAGCACGAAGAAACCGCCGc -3' miRNA: 3'- -UACAGCGUUCGUGCUUCUUUGGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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