Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3509 | 3' | -52.7 | NC_001623.1 | + | 12820 | 0.66 | 0.965483 |
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Target: 5'- aCGUCguuuauCGCCACUAgUAGUUgaucGCUGGAAa -3' miRNA: 3'- -GCAG------GCGGUGGU-AUCAGa---UGACCUUg -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 18434 | 0.66 | 0.962032 |
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Target: 5'- gCGUCCGUguUACUAUcaaaaguGUC-ACUGGGACa -3' miRNA: 3'- -GCAGGCG--GUGGUAu------CAGaUGACCUUG- -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 128750 | 0.67 | 0.954428 |
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Target: 5'- uGUCCGaCGCUAUAacGUUUGC-GGAACa -3' miRNA: 3'- gCAGGCgGUGGUAU--CAGAUGaCCUUG- -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 38049 | 0.67 | 0.936285 |
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Target: 5'- gCG-CCG-CGCCAUGGUCcACaGGGGCg -3' miRNA: 3'- -GCaGGCgGUGGUAUCAGaUGaCCUUG- -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 132745 | 0.67 | 0.93578 |
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Target: 5'- -uUCCGCCAUCAUucgCUGCUguuccauuuguauGGAGCg -3' miRNA: 3'- gcAGGCGGUGGUAucaGAUGA-------------CCUUG- -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 66117 | 0.72 | 0.748971 |
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Target: 5'- -uUCCGCCACCAUG---UACUGGAc- -3' miRNA: 3'- gcAGGCGGUGGUAUcagAUGACCUug -5' |
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| 3509 | 3' | -52.7 | NC_001623.1 | + | 95691 | 1.09 | 0.004958 |
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Target: 5'- gCGUCCGCCACCAUAGUCUACUGGAACc -3' miRNA: 3'- -GCAGGCGGUGGUAUCAGAUGACCUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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