Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
351 | 3' | -48.3 | AC_000011.1 | + | 25969 | 0.66 | 0.945284 |
Target: 5'- aGCGCCggGugAAGGAGAuguuGCCcccgcAGGCc -3' miRNA: 3'- gCGCGG--CugUUCCUUUuuu-UGG-----UCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 11335 | 0.66 | 0.945284 |
Target: 5'- uCGCGCgCGAgGAGGugacccuGGGCCuGAUg -3' miRNA: 3'- -GCGCG-GCUgUUCCuuuu---UUUGGuCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 7137 | 0.66 | 0.939963 |
Target: 5'- uGCGCCuACAAGGccGucAACCAGu- -3' miRNA: 3'- gCGCGGcUGUUCCuuUuuUUGGUCug -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 15850 | 0.66 | 0.939963 |
Target: 5'- gGCGCaCGggacGCAGGGccaugcucAGGGcgGCCAGACg -3' miRNA: 3'- gCGCG-GC----UGUUCC--------UUUUuuUGGUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 11177 | 0.66 | 0.93432 |
Target: 5'- gGUGCUgaggGACGAGGAuuucGAGgCGGACg -3' miRNA: 3'- gCGCGG----CUGUUCCUuuu-UUUgGUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 6434 | 0.66 | 0.928352 |
Target: 5'- gGgGUCGGCAucgauGGuGAAGAuGCCGGGCa -3' miRNA: 3'- gCgCGGCUGUu----CC-UUUUUuUGGUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 5524 | 0.66 | 0.928352 |
Target: 5'- uCGgGCUGGuCGGGGucAAAAACCAGu- -3' miRNA: 3'- -GCgCGGCU-GUUCCuuUUUUUGGUCug -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 20406 | 0.66 | 0.922058 |
Target: 5'- gGUGCCGuCGAGGuaggggauGGAGCCcgaguAGACg -3' miRNA: 3'- gCGCGGCuGUUCCuuu-----UUUUGG-----UCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 18688 | 0.66 | 0.922058 |
Target: 5'- gGUGCCGGaguAGGGuuuGAAGCUAGGg -3' miRNA: 3'- gCGCGGCUgu-UCCUuu-UUUUGGUCUg -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 9281 | 0.67 | 0.915439 |
Target: 5'- aGUGgUGGCGGGGGAGGGGGCCu-GCg -3' miRNA: 3'- gCGCgGCUGUUCCUUUUUUUGGucUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 7549 | 0.67 | 0.893654 |
Target: 5'- gGgGuuGGCGcggAGGAAGGAAGuCCAGAg -3' miRNA: 3'- gCgCggCUGU---UCCUUUUUUU-GGUCUg -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 25535 | 0.67 | 0.893654 |
Target: 5'- cCGC-CCGugGAGGAuuuggaGGAAGACUGGGa -3' miRNA: 3'- -GCGcGGCugUUCCU------UUUUUUGGUCUg -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 5228 | 0.67 | 0.893654 |
Target: 5'- gCGCGCCGggcugggcgcuuGCGAGGGugcgcuUCAGGCu -3' miRNA: 3'- -GCGCGGC------------UGUUCCUuuuuuuGGUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 17363 | 0.67 | 0.893654 |
Target: 5'- cCGCGgCGAUGAuGGGGAucAGCgCGGGCa -3' miRNA: 3'- -GCGCgGCUGUU-CCUUUuuUUG-GUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 3695 | 0.68 | 0.877566 |
Target: 5'- aGCGCCGuGCGcGGAAu--GGCCauGGGCg -3' miRNA: 3'- gCGCGGC-UGUuCCUUuuuUUGG--UCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 17186 | 0.68 | 0.869071 |
Target: 5'- aCGgGCUacCGAGGAAGAAAACCGcGCc -3' miRNA: 3'- -GCgCGGcuGUUCCUUUUUUUGGUcUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 24500 | 0.68 | 0.860287 |
Target: 5'- cCGCGUCGGCGAaGAAGc-GGCgCAGGCa -3' miRNA: 3'- -GCGCGGCUGUUcCUUUuuUUG-GUCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 33637 | 0.68 | 0.851222 |
Target: 5'- uCGgGUCGGuCAGGGAGAuGAAACCcuccGGGCa -3' miRNA: 3'- -GCgCGGCU-GUUCCUUU-UUUUGG----UCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 329 | 0.68 | 0.851222 |
Target: 5'- uCGCGUuuaUGGCAGGGAGGAGuauuuGCCgaGGGCc -3' miRNA: 3'- -GCGCG---GCUGUUCCUUUUUu----UGG--UCUG- -5' |
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351 | 3' | -48.3 | AC_000011.1 | + | 13610 | 0.68 | 0.841887 |
Target: 5'- gGCGUCG-CAGGGGGccacGAGCCGGGg -3' miRNA: 3'- gCGCGGCuGUUCCUUuu--UUUGGUCUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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