Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
351 | 5' | -57.9 | AC_000011.1 | + | 9953 | 0.66 | 0.448218 |
Target: 5'- cUCGCCGAGCgCgUCgucgCCGACcugGCgCUCg -3' miRNA: 3'- -AGCGGUUCGaGgAGa---GGUUG---CGgGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 26286 | 0.66 | 0.438254 |
Target: 5'- aCGCCgAGGCU-CUCUUCAacaaguacuGCGCgCUCa -3' miRNA: 3'- aGCGG-UUCGAgGAGAGGU---------UGCGgGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 4795 | 0.66 | 0.418714 |
Target: 5'- -gGCCcAGCUCaUCUCCccCGCCC-Cg -3' miRNA: 3'- agCGGuUCGAGgAGAGGuuGCGGGaG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 27490 | 0.66 | 0.409145 |
Target: 5'- -aGaCCGAGCUCCagCUCCAGuguaaGCCC-Ca -3' miRNA: 3'- agC-GGUUCGAGGa-GAGGUUg----CGGGaG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 9519 | 0.67 | 0.390421 |
Target: 5'- aUCGUCAGcGC-CCUCUCCc-UGCCCa- -3' miRNA: 3'- -AGCGGUU-CGaGGAGAGGuuGCGGGag -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 31610 | 0.67 | 0.372266 |
Target: 5'- aUCGCCucuccuacGAGCUCCUgCagCAGCGCCa-- -3' miRNA: 3'- -AGCGG--------UUCGAGGA-GagGUUGCGGgag -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 5503 | 0.68 | 0.346131 |
Target: 5'- aCGCCcccGAGUccgucuUCCUCgcccCCAagcucuACGCCCUCa -3' miRNA: 3'- aGCGG---UUCG------AGGAGa---GGU------UGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 9064 | 0.68 | 0.345282 |
Target: 5'- gUCGCCcAGCgCCUCcaaacguUCCAugGCCUc- -3' miRNA: 3'- -AGCGGuUCGaGGAG-------AGGUugCGGGag -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 10250 | 0.68 | 0.337716 |
Target: 5'- aUGCuCGAGgaCCUCgcgcCCGGCGCCCcCg -3' miRNA: 3'- aGCG-GUUCgaGGAGa---GGUUGCGGGaG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 21477 | 0.68 | 0.337716 |
Target: 5'- cCGCCugcgGGCUCUUCugcugcauguUCCugcACGCCUUCg -3' miRNA: 3'- aGCGGu---UCGAGGAG----------AGGu--UGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 20304 | 0.68 | 0.337716 |
Target: 5'- cUCGCUggGCUCCgggUUCGAC-CCCUa -3' miRNA: 3'- -AGCGGuuCGAGGag-AGGUUGcGGGAg -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 15441 | 0.68 | 0.337716 |
Target: 5'- gCGCCGcGCgUCCUCUCgAGcCGCaCCUUc -3' miRNA: 3'- aGCGGUuCG-AGGAGAGgUU-GCG-GGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 15694 | 0.68 | 0.329451 |
Target: 5'- cCGCCGcGC-CCgUCUCCAccguggACGCCgUCa -3' miRNA: 3'- aGCGGUuCGaGG-AGAGGU------UGCGGgAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 23958 | 0.68 | 0.321336 |
Target: 5'- gCGCCAAccGCaCC-CgcgCCGACGCCCUUu -3' miRNA: 3'- aGCGGUU--CGaGGaGa--GGUUGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 8891 | 0.69 | 0.283017 |
Target: 5'- aCGCCuucucgcAGCUCaugggccgcaUCUCCAACGaCCUCg -3' miRNA: 3'- aGCGGu------UCGAGg---------AGAGGUUGCgGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 15148 | 0.69 | 0.275801 |
Target: 5'- gCGCCGAGCUCCUgcccgucuaCUCCAAgaGCUucUUCa -3' miRNA: 3'- aGCGGUUCGAGGA---------GAGGUUg-CGG--GAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 25131 | 0.69 | 0.275801 |
Target: 5'- cUCGagCAGGC-CCUCgccgCUGACGUCCUCg -3' miRNA: 3'- -AGCg-GUUCGaGGAGa---GGUUGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 9045 | 0.69 | 0.268733 |
Target: 5'- aUCGCCAccacGCUcaacuaCCUCUaCCAGCGCCuCUg -3' miRNA: 3'- -AGCGGUu---CGA------GGAGA-GGUUGCGG-GAg -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 15579 | 0.7 | 0.235573 |
Target: 5'- gUGCgCGGGCacuUCCgcgCUCCcuggGGCGCCCUCa -3' miRNA: 3'- aGCG-GUUCG---AGGa--GAGG----UUGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 7507 | 0.71 | 0.21737 |
Target: 5'- gUCGCUGAGC-CCgugcugCUCgAGCGCCCa- -3' miRNA: 3'- -AGCGGUUCGaGGa-----GAGgUUGCGGGag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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