Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
351 | 5' | -57.9 | AC_000011.1 | + | 25131 | 0.69 | 0.275801 |
Target: 5'- cUCGagCAGGC-CCUCgccgCUGACGUCCUCg -3' miRNA: 3'- -AGCg-GUUCGaGGAGa---GGUUGCGGGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 26286 | 0.66 | 0.438254 |
Target: 5'- aCGCCgAGGCU-CUCUUCAacaaguacuGCGCgCUCa -3' miRNA: 3'- aGCGG-UUCGAgGAGAGGU---------UGCGgGAG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 27490 | 0.66 | 0.409145 |
Target: 5'- -aGaCCGAGCUCCagCUCCAGuguaaGCCC-Ca -3' miRNA: 3'- agC-GGUUCGAGGa-GAGGUUg----CGGGaG- -5' |
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351 | 5' | -57.9 | AC_000011.1 | + | 31610 | 0.67 | 0.372266 |
Target: 5'- aUCGCCucuccuacGAGCUCCUgCagCAGCGCCa-- -3' miRNA: 3'- -AGCGG--------UUCGAGGA-GagGUUGCGGgag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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