Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3512 | 3' | -55.5 | NC_001623.1 | + | 120746 | 0.66 | 0.873381 |
Target: 5'- cUUGCUGAaaGCCGUuugacggccaGCGCUuuGUCgGCCa -3' miRNA: 3'- -AACGACUa-CGGCA----------CGUGGu-UAGgCGG- -5' |
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3512 | 3' | -55.5 | NC_001623.1 | + | 55637 | 0.66 | 0.873381 |
Target: 5'- -aGUUGAUGuuGUGCGUCAAcguguugauaucUCgGCCg -3' miRNA: 3'- aaCGACUACggCACGUGGUU------------AGgCGG- -5' |
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3512 | 3' | -55.5 | NC_001623.1 | + | 12097 | 0.67 | 0.813122 |
Target: 5'- -gGUggacggGGUGCCGcgccacgGCGCCGAccgugucaaaguuuUCCGCCa -3' miRNA: 3'- aaCGa-----CUACGGCa------CGUGGUU--------------AGGCGG- -5' |
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3512 | 3' | -55.5 | NC_001623.1 | + | 31073 | 0.68 | 0.778855 |
Target: 5'- -gGCUGuccUGCUGUGUGCCcGUCgCGCg -3' miRNA: 3'- aaCGACu--ACGGCACGUGGuUAG-GCGg -5' |
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3512 | 3' | -55.5 | NC_001623.1 | + | 103780 | 0.69 | 0.729624 |
Target: 5'- ---gUGAcGCUGUGCACUAcaCCGCCa -3' miRNA: 3'- aacgACUaCGGCACGUGGUuaGGCGG- -5' |
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3512 | 3' | -55.5 | NC_001623.1 | + | 108642 | 1.1 | 0.001874 |
Target: 5'- uUUGCUGAUGCCGUGCACCAAUCCGCCg -3' miRNA: 3'- -AACGACUACGGCACGUGGUUAGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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