Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3513 | 3' | -55 | NC_001623.1 | + | 90751 | 0.66 | 0.92946 |
Target: 5'- uGCGgaagacgacUUGUUGCCGacuCGCAGCAGcaaACGUg -3' miRNA: 3'- gCGCa--------AACAGUGGC---GCGUCGUC---UGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 68999 | 0.66 | 0.928932 |
Target: 5'- gGUGUUUGacggCACCGCGUGcgugccagccaccGCGGACa- -3' miRNA: 3'- gCGCAAACa---GUGGCGCGU-------------CGUCUGcg -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 87644 | 0.66 | 0.924077 |
Target: 5'- aCGCGgc-GUagGCUGCGCGGacgcuGGCGCg -3' miRNA: 3'- -GCGCaaaCAg-UGGCGCGUCgu---CUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 12646 | 0.66 | 0.922415 |
Target: 5'- uGCGUaauaaaCGCCGCcauuuuuuuaugacGCAGCAcGGCGCg -3' miRNA: 3'- gCGCAaaca--GUGGCG--------------CGUCGU-CUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 2307 | 0.66 | 0.917875 |
Target: 5'- cCGCGUUgacUCGCUGCaccucgaGCAGUucguuGACGCc -3' miRNA: 3'- -GCGCAAac-AGUGGCG-------CGUCGu----CUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 35818 | 0.66 | 0.912581 |
Target: 5'- gGUGU---UCACgGUGCGGCgcuacaacAGACGCg -3' miRNA: 3'- gCGCAaacAGUGgCGCGUCG--------UCUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 85636 | 0.69 | 0.819118 |
Target: 5'- gCGCGUUuaauauugugccugaUGUCGCgcaUGuCGuCGGCGGGCGCu -3' miRNA: 3'- -GCGCAA---------------ACAGUG---GC-GC-GUCGUCUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 120785 | 0.7 | 0.769843 |
Target: 5'- aGCGUggGUUA-CGCGCAGCccGCGUg -3' miRNA: 3'- gCGCAaaCAGUgGCGCGUCGucUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 36609 | 0.7 | 0.740797 |
Target: 5'- uGCGagUGUCAaccUCGCGCAGCAauUGCu -3' miRNA: 3'- gCGCaaACAGU---GGCGCGUCGUcuGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 87685 | 0.72 | 0.639104 |
Target: 5'- cCGCGUUUccCGCCGCGUAcugAGACGCu -3' miRNA: 3'- -GCGCAAAcaGUGGCGCGUcg-UCUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 23720 | 0.72 | 0.628759 |
Target: 5'- gCGCGcUUGUCuagaaCGCGCAGCuGuuuGCGCg -3' miRNA: 3'- -GCGCaAACAGug---GCGCGUCGuC---UGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 102219 | 0.75 | 0.487477 |
Target: 5'- uCGCGaucgUUGUUcagaaagaaGCCGCGCAaucuuaacgcuGCAGGCGCg -3' miRNA: 3'- -GCGCa---AACAG---------UGGCGCGU-----------CGUCUGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 87823 | 0.75 | 0.468394 |
Target: 5'- aCGCGgacgucGUCGCCGCGCcAGCGucCGCg -3' miRNA: 3'- -GCGCaaa---CAGUGGCGCG-UCGUcuGCG- -5' |
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3513 | 3' | -55 | NC_001623.1 | + | 115102 | 1.12 | 0.002214 |
Target: 5'- aCGCGUUUGUCACCGCGCAGCAGACGCc -3' miRNA: 3'- -GCGCAAACAGUGGCGCGUCGUCUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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