Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3513 | 5' | -55.5 | NC_001623.1 | + | 37339 | 0.66 | 0.877114 |
Target: 5'- aGGGCGCUuguUGGUGCggCGGCGgaucgGGCAGUu -3' miRNA: 3'- -CCCGCGGu--AUCACGg-GUUGC-----UCGUUA- -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 88985 | 0.66 | 0.86965 |
Target: 5'- -cGCGUCGagGGUGCUCAGCGAGgAc- -3' miRNA: 3'- ccCGCGGUa-UCACGGGUUGCUCgUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 68574 | 0.66 | 0.865064 |
Target: 5'- uGGGCGCCAcgUGGUgggcgaauGCCCcGCGAauaaaauauuugaucGCAAc -3' miRNA: 3'- -CCCGCGGU--AUCA--------CGGGuUGCU---------------CGUUa -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 76472 | 0.67 | 0.8291 |
Target: 5'- cGGCGCCAUA---CCC-ACGGGCAc- -3' miRNA: 3'- cCCGCGGUAUcacGGGuUGCUCGUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 64435 | 0.67 | 0.817746 |
Target: 5'- gGGGCGCgAUAGUGUUUugcaucauguuaggGACGGGUAu- -3' miRNA: 3'- -CCCGCGgUAUCACGGG--------------UUGCUCGUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 121753 | 0.67 | 0.811507 |
Target: 5'- cGGCGCauaAUucgaaaucacaaAGaGCCCAACGAGCGc- -3' miRNA: 3'- cCCGCGg--UA------------UCaCGGGUUGCUCGUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 6564 | 0.68 | 0.80245 |
Target: 5'- cGGCGCCugcgccGGUGCCCAcaauCGuGCGc- -3' miRNA: 3'- cCCGCGGua----UCACGGGUu---GCuCGUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 76633 | 0.68 | 0.793231 |
Target: 5'- uGGCGCU--AGUGCCCGugGguauGGCGc- -3' miRNA: 3'- cCCGCGGuaUCACGGGUugC----UCGUua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 38170 | 0.69 | 0.754922 |
Target: 5'- -uGCGUggaCGUGGUGCCCGACGGGa--- -3' miRNA: 3'- ccCGCG---GUAUCACGGGUUGCUCguua -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 55529 | 0.7 | 0.652562 |
Target: 5'- cGGCGCCc--GUGCCC-GCGAuGCAAc -3' miRNA: 3'- cCCGCGGuauCACGGGuUGCU-CGUUa -5' |
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3513 | 5' | -55.5 | NC_001623.1 | + | 115068 | 1.08 | 0.002877 |
Target: 5'- cGGGCGCCAUAGUGCCCAACGAGCAAUu -3' miRNA: 3'- -CCCGCGGUAUCACGGGUUGCUCGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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