Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3514 | 3' | -54.3 | NC_001623.1 | + | 89632 | 0.66 | 0.931637 |
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Target: 5'- cGCA-AUcGCCGCCGCGGguaacaUCCAGCGa -3' miRNA: 3'- uCGUaUGaUGGCGGUGCU------GGGUUGCa -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 105676 | 0.67 | 0.914861 |
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Target: 5'- gGGCGUcGCcaugUACgGCCGCGGCuggaCCGGCGUa -3' miRNA: 3'- -UCGUA-UG----AUGgCGGUGCUG----GGUUGCA- -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 54462 | 0.67 | 0.895854 |
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Target: 5'- aAGCGUuacgauUUGCCgGCUGCGGCgCCGACGa -3' miRNA: 3'- -UCGUAu-----GAUGG-CGGUGCUG-GGUUGCa -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 32893 | 0.68 | 0.843322 |
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Target: 5'- cGUAUGCUcaACUGU--UGACCCAACGUg -3' miRNA: 3'- uCGUAUGA--UGGCGguGCUGGGUUGCA- -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 121647 | 0.69 | 0.834962 |
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Target: 5'- uGCAgcGCUACCGaagcaugugcaCCA-GACCCGACGUg -3' miRNA: 3'- uCGUa-UGAUGGC-----------GGUgCUGGGUUGCA- -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 124115 | 0.7 | 0.771519 |
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Target: 5'- -aUAUACUACCGCCGC-ACCCuucuAACGc -3' miRNA: 3'- ucGUAUGAUGGCGGUGcUGGG----UUGCa -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 120765 | 0.71 | 0.691437 |
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Target: 5'- cGCGUGCUACCaCCugGACagggCGGCGg -3' miRNA: 3'- uCGUAUGAUGGcGGugCUGg---GUUGCa -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 126738 | 0.73 | 0.587169 |
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Target: 5'- gGGCAUGCUAgCGCaCACGgacaauggACCCGACa- -3' miRNA: 3'- -UCGUAUGAUgGCG-GUGC--------UGGGUUGca -5' |
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| 3514 | 3' | -54.3 | NC_001623.1 | + | 120345 | 1.08 | 0.004083 |
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Target: 5'- cAGCAUACUACCGCCACGACCCAACGUg -3' miRNA: 3'- -UCGUAUGAUGGCGGUGCUGGGUUGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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