Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3515 | 5' | -56 | NC_001623.1 | + | 108479 | 0.66 | 0.859185 |
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Target: 5'- uGCC-GGCgGaUUGGuGCACGGCAucaGCAa -3' miRNA: 3'- -CGGuUCGgC-AACCuCGUGUCGU---CGUa -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 65942 | 0.66 | 0.842917 |
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Target: 5'- gGCCGAGCUGgcgacGcGAGC-CGGCAaGCAg -3' miRNA: 3'- -CGGUUCGGCaa---C-CUCGuGUCGU-CGUa -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 109395 | 0.68 | 0.710218 |
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Target: 5'- aGCUGAucGaCCGUUGGGGCAguGaCAGCGa -3' miRNA: 3'- -CGGUU--C-GGCAACCUCGUguC-GUCGUa -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 131940 | 0.69 | 0.68845 |
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Target: 5'- aGCCAucGCCGUcaGGAGCggcgucaGgAGCAGCGUg -3' miRNA: 3'- -CGGUu-CGGCAa-CCUCG-------UgUCGUCGUA- -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 11253 | 0.7 | 0.636958 |
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Target: 5'- cGUCAAaCCGUUGGAcgACGGCGGCGg -3' miRNA: 3'- -CGGUUcGGCAACCUcgUGUCGUCGUa -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 89802 | 0.71 | 0.542799 |
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Target: 5'- gGCCAGGUCGcUGGAuGUuacccGCGGCGGCGa -3' miRNA: 3'- -CGGUUCGGCaACCU-CG-----UGUCGUCGUa -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 35755 | 0.73 | 0.472822 |
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Target: 5'- gGCCAugcGCCGUUGGuaaaaCGCAGCGGCc- -3' miRNA: 3'- -CGGUu--CGGCAACCuc---GUGUCGUCGua -5' |
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| 3515 | 5' | -56 | NC_001623.1 | + | 122380 | 1.07 | 0.002589 |
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Target: 5'- gGCCAAGCCGUUGGAGCACAGCAGCAUu -3' miRNA: 3'- -CGGUUCGGCAACCUCGUGUCGUCGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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