Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3516 | 3' | -45.2 | NC_001623.1 | + | 2556 | 0.66 | 0.999991 |
Target: 5'- aCAGaUACUUGUGCgucgcGUgCGGCaCGCc -3' miRNA: 3'- aGUUaAUGAAUAUGa----UAgGCCGcGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 65953 | 0.66 | 0.999988 |
Target: 5'- uUCAAUUACaUGgcCgagCUGGCGaCGCg -3' miRNA: 3'- -AGUUAAUGaAUauGauaGGCCGC-GCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 130235 | 0.66 | 0.999988 |
Target: 5'- uUUAAaUAUUgAUGCgcUCCacGGCGCGCg -3' miRNA: 3'- -AGUUaAUGAaUAUGauAGG--CCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 10668 | 0.66 | 0.999988 |
Target: 5'- aUAAUUAUa---GCUGUaCGGUGCGCg -3' miRNA: 3'- aGUUAAUGaauaUGAUAgGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 53383 | 0.66 | 0.999983 |
Target: 5'- aUAAUaACcguaUAUGCUAUUggCGGUGCGCu -3' miRNA: 3'- aGUUAaUGa---AUAUGAUAG--GCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 122631 | 0.66 | 0.999977 |
Target: 5'- aUCAGUaucaaUUGUGC--UCCGGCGCa- -3' miRNA: 3'- -AGUUAaug--AAUAUGauAGGCCGCGcg -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 100896 | 0.67 | 0.999955 |
Target: 5'- uUCGAUUACUauuucggACggcgGUUcaauaagCGGCGCGCg -3' miRNA: 3'- -AGUUAAUGAaua----UGa---UAG-------GCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 109125 | 0.67 | 0.999941 |
Target: 5'- aCGAUUugUUGcAC-GUgUGGUGCGCa -3' miRNA: 3'- aGUUAAugAAUaUGaUAgGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 126708 | 0.67 | 0.999941 |
Target: 5'- gCAGUgUACUUGUugUAugcaaauaaaUCucgauaaagggCGGCGCGCg -3' miRNA: 3'- aGUUA-AUGAAUAugAU----------AG-----------GCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 120838 | 0.67 | 0.999941 |
Target: 5'- gUCGAUUAgUaUAUGCggaaacCUGGUGCGCc -3' miRNA: 3'- -AGUUAAUgA-AUAUGaua---GGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 119925 | 0.67 | 0.999941 |
Target: 5'- aCAauGUUGCUUAgcACgacUCGGCGCGUg -3' miRNA: 3'- aGU--UAAUGAAUa-UGauaGGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 34690 | 0.67 | 0.999921 |
Target: 5'- cCAA-UGCUUGUugUGgaUCGGUGUGCc -3' miRNA: 3'- aGUUaAUGAAUAugAUa-GGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 81227 | 0.68 | 0.999702 |
Target: 5'- aCGAgucGCUUGUGacg-CCGGUGCGUu -3' miRNA: 3'- aGUUaa-UGAAUAUgauaGGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 1901 | 0.69 | 0.999621 |
Target: 5'- aCAG-UGCUcauUACUAaaUCCGGCGUGa -3' miRNA: 3'- aGUUaAUGAau-AUGAU--AGGCCGCGCg -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 32253 | 0.69 | 0.999521 |
Target: 5'- aUUGAUUACacgAUugUGUUgGGCgGCGCg -3' miRNA: 3'- -AGUUAAUGaa-UAugAUAGgCCG-CGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 62716 | 0.7 | 0.998854 |
Target: 5'- uUCGAUUAUUaucgccGCUuguugCCGGCGCGUc -3' miRNA: 3'- -AGUUAAUGAaua---UGAua---GGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 62300 | 0.71 | 0.997521 |
Target: 5'- aCAAUUcgGCUuaaUAUACUcgUCGGCGCa- -3' miRNA: 3'- aGUUAA--UGA---AUAUGAuaGGCCGCGcg -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 110416 | 0.71 | 0.996475 |
Target: 5'- aUCAGUUcuugcaACUUGUuaaUGUCUGGCGCagGCg -3' miRNA: 3'- -AGUUAA------UGAAUAug-AUAGGCCGCG--CG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 41330 | 0.71 | 0.995089 |
Target: 5'- aCGAcggACUUGUGCUGUacauggCCGGUGUGUu -3' miRNA: 3'- aGUUaa-UGAAUAUGAUA------GGCCGCGCG- -5' |
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3516 | 3' | -45.2 | NC_001623.1 | + | 84778 | 0.73 | 0.984602 |
Target: 5'- uUCAAUUaGCgucaagcUGCUGaCCGGCGUGCa -3' miRNA: 3'- -AGUUAA-UGaau----AUGAUaGGCCGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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