Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3518 | 3' | -49.3 | NC_001623.1 | + | 119950 | 0.66 | 0.997295 |
Target: 5'- aACGgUCGAGCGcCuCGAaaUGUUCGACAc -3' miRNA: 3'- -UGCgAGUUUGCuGuGCU--ACAGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 77079 | 0.66 | 0.995546 |
Target: 5'- cGCGC-CcGACGACGCGcagcuaaacgugAUGUgCAACAu -3' miRNA: 3'- -UGCGaGuUUGCUGUGC------------UACAgGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 89567 | 0.66 | 0.995546 |
Target: 5'- aACGCUCAguuuggaagaaaAAauaGACACGuUGaCCAGCAu -3' miRNA: 3'- -UGCGAGU------------UUg--CUGUGCuACaGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 119608 | 0.67 | 0.99479 |
Target: 5'- aGCGUcaauaGAAUGAgCACGAUG-CCGACAa -3' miRNA: 3'- -UGCGag---UUUGCU-GUGCUACaGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 65937 | 0.67 | 0.99479 |
Target: 5'- -aGCU--GGCGACGCGAg--CCGGCAa -3' miRNA: 3'- ugCGAguUUGCUGUGCUacaGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 38109 | 0.67 | 0.99479 |
Target: 5'- cCGaCUCAAACGACACcGUGUU--GCAu -3' miRNA: 3'- uGC-GAGUUUGCUGUGcUACAGguUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 67476 | 0.67 | 0.990668 |
Target: 5'- uGCGUUUggGCGACgACGuGUGUaCAGCAg -3' miRNA: 3'- -UGCGAGuuUGCUG-UGC-UACAgGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 87513 | 0.67 | 0.990668 |
Target: 5'- -gGUUCAAACgGACACGcUGUUgGACGc -3' miRNA: 3'- ugCGAGUUUG-CUGUGCuACAGgUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 38425 | 0.68 | 0.986158 |
Target: 5'- uGCGCuUCAAAUGACAaaucguUGGUGUugaCCGACGu -3' miRNA: 3'- -UGCG-AGUUUGCUGU------GCUACA---GGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 105361 | 0.69 | 0.980139 |
Target: 5'- gACGCUUGAGCGGCGC--UGUCaAACGu -3' miRNA: 3'- -UGCGAGUUUGCUGUGcuACAGgUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 30412 | 0.7 | 0.962586 |
Target: 5'- cACGCUCAAGC-ACACGAUGaaC-ACAg -3' miRNA: 3'- -UGCGAGUUUGcUGUGCUACagGuUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 99183 | 0.7 | 0.958853 |
Target: 5'- cCGCUCGAACaaucGCACGc-GUCCGGCGa -3' miRNA: 3'- uGCGAGUUUGc---UGUGCuaCAGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 65287 | 0.7 | 0.950637 |
Target: 5'- gGCGCUCAacGACGAUAC--UG-CCAGCGa -3' miRNA: 3'- -UGCGAGU--UUGCUGUGcuACaGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 49008 | 0.72 | 0.907308 |
Target: 5'- uCGCUCAu-CGACuuGAuauUGUCCGACAc -3' miRNA: 3'- uGCGAGUuuGCUGugCU---ACAGGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 76416 | 0.76 | 0.753828 |
Target: 5'- cACGCgUCGAACGACACGAUGgacgcgaaaaUgCAGCGa -3' miRNA: 3'- -UGCG-AGUUUGCUGUGCUAC----------AgGUUGU- -5' |
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3518 | 3' | -49.3 | NC_001623.1 | + | 30209 | 1.09 | 0.011605 |
Target: 5'- uACGCUCAAACGACACGAUGUCCAACAu -3' miRNA: 3'- -UGCGAGUUUGCUGUGCUACAGGUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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