Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3519 | 3' | -59.6 | NC_001623.1 | + | 105507 | 0.66 | 0.714253 |
Target: 5'- ---uAC-GCCGGUCCAGCcGCGGcCGUa -3' miRNA: 3'- uuuuUGcCGGCCGGGUUGcCGCC-GCA- -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 131817 | 0.66 | 0.690321 |
Target: 5'- uGAAGACGGCUGGCUgcgacugcuugaaGACGGCugGGCu- -3' miRNA: 3'- -UUUUUGCCGGCCGGg------------UUGCCG--CCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 95225 | 0.66 | 0.684291 |
Target: 5'- aGAGAACGcGCCGGCUUAugaAgGGCGaCGUg -3' miRNA: 3'- -UUUUUGC-CGGCCGGGU---UgCCGCcGCA- -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 6277 | 0.67 | 0.664088 |
Target: 5'- uGGAGCGGgCGGCaauucagacaUCGAUGGUGGUGg -3' miRNA: 3'- uUUUUGCCgGCCG----------GGUUGCCGCCGCa -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 37354 | 0.67 | 0.661047 |
Target: 5'- --cGGCGaCCGGgcCCCAgggcgcuuguugguGCGGCGGCGg -3' miRNA: 3'- uuuUUGCcGGCC--GGGU--------------UGCCGCCGCa -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 40705 | 0.67 | 0.649881 |
Target: 5'- ---cGCGGCgGGUCCAACuugccgcaaaacuGCGGCGa -3' miRNA: 3'- uuuuUGCCGgCCGGGUUGc------------CGCCGCa -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 120762 | 0.67 | 0.633612 |
Target: 5'- --uGACGGCCagcgcuuugucGGCCaauuuCGGCGGCa- -3' miRNA: 3'- uuuUUGCCGG-----------CCGGguu--GCCGCCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 42501 | 0.67 | 0.613276 |
Target: 5'- cAAGACGGCCGacgacgacGCgUAcACGGUGGCGa -3' miRNA: 3'- uUUUUGCCGGC--------CGgGU-UGCCGCCGCa -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 100882 | 0.68 | 0.603125 |
Target: 5'- -cGGACGG-CGGUUCAauaaGCGGCGcGCGUg -3' miRNA: 3'- uuUUUGCCgGCCGGGU----UGCCGC-CGCA- -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 131884 | 0.68 | 0.602111 |
Target: 5'- --cGACGGCUGagagacaGCCUguGGCGGCGGCu- -3' miRNA: 3'- uuuUUGCCGGC-------CGGG--UUGCCGCCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 101658 | 0.68 | 0.559805 |
Target: 5'- ---uGCGGUCGcuuugucuaaccauGCCCGuguCGGCGGUGUg -3' miRNA: 3'- uuuuUGCCGGC--------------CGGGUu--GCCGCCGCA- -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 89805 | 0.69 | 0.523239 |
Target: 5'- ---uGCGGCCaGGUcgcuggauguuaCCcGCGGCGGCGa -3' miRNA: 3'- uuuuUGCCGG-CCG------------GGuUGCCGCCGCa -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 65711 | 0.69 | 0.502894 |
Target: 5'- -uGAGCGGCCGGCgaauugaCCGcguUGGCGGCc- -3' miRNA: 3'- uuUUUGCCGGCCG-------GGUu--GCCGCCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 37360 | 0.71 | 0.429817 |
Target: 5'- -cGAugGGCUGGCaCCgGACGGCaGGCa- -3' miRNA: 3'- uuUUugCCGGCCG-GG-UUGCCG-CCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 11930 | 0.72 | 0.370566 |
Target: 5'- ---cACGGUCGGCgCCGugGcGCGGCa- -3' miRNA: 3'- uuuuUGCCGGCCG-GGUugC-CGCCGca -5' |
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3519 | 3' | -59.6 | NC_001623.1 | + | 34110 | 1.05 | 0.001938 |
Target: 5'- aAAAAACGGCCGGCCCAACGGCGGCGUg -3' miRNA: 3'- -UUUUUGCCGGCCGGGUUGCCGCCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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