Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
352 | 3' | -61.1 | AC_000011.1 | + | 4498 | 0.66 | 0.360832 |
Target: 5'- gCCCGuGg-GCGgCGGCCUGGGCaaagacguuUCGGGg -3' miRNA: 3'- -GGGU-CagCGUgGUUGGACCCG---------GGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 1489 | 0.66 | 0.344351 |
Target: 5'- aUguGUgGCACUuaguGCCUGGGgcguuUCCGGGu -3' miRNA: 3'- gGguCAgCGUGGu---UGGACCC-----GGGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 18134 | 0.66 | 0.344351 |
Target: 5'- cCCCGGgucgCGCGCgGGCCacGGCCaccggcggcaGGGg -3' miRNA: 3'- -GGGUCa---GCGUGgUUGGacCCGGg---------CCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 13671 | 0.66 | 0.336316 |
Target: 5'- cCCCGGcUCGUgGCCc-CCUGcgacGCCCGGGg -3' miRNA: 3'- -GGGUC-AGCG-UGGuuGGACc---CGGGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 11328 | 0.67 | 0.313038 |
Target: 5'- aCCCuGaUCGCGCgCGAggaggugacCCUGGGCCUGa- -3' miRNA: 3'- -GGGuC-AGCGUG-GUU---------GGACCCGGGCcc -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 12460 | 0.67 | 0.305556 |
Target: 5'- aCCCAGcC-CGCCAACgUGc-CCCGGGg -3' miRNA: 3'- -GGGUCaGcGUGGUUGgACccGGGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 11042 | 0.67 | 0.29821 |
Target: 5'- cCCCAGgCGCGCCAGCCccucGCCCu-- -3' miRNA: 3'- -GGGUCaGCGUGGUUGGacc-CGGGccc -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 22675 | 0.67 | 0.276998 |
Target: 5'- uCCCAGaUCGC-CCAcaagaaCUGGGagugcaCCGGGu -3' miRNA: 3'- -GGGUC-AGCGuGGUug----GACCCg-----GGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 9950 | 0.67 | 0.276998 |
Target: 5'- gCCGagcgcGUCGuCGCCGACCUGGcGCUCGu- -3' miRNA: 3'- gGGU-----CAGC-GUGGUUGGACC-CGGGCcc -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 16786 | 0.68 | 0.263537 |
Target: 5'- uCCaCGGUCuGCAC--GCCcaGGCCCGGGg -3' miRNA: 3'- -GG-GUCAG-CGUGguUGGacCCGGGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 23969 | 0.68 | 0.257007 |
Target: 5'- aCCCGcGcCGaCGCCcuuuucAACCUGGGuCCCGGc -3' miRNA: 3'- -GGGU-CaGC-GUGG------UUGGACCC-GGGCCc -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 5782 | 0.71 | 0.165221 |
Target: 5'- uCCU--UCGUGCUGGCCUGGaCCCGGGc -3' miRNA: 3'- -GGGucAGCGUGGUUGGACCcGGGCCC- -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 30917 | 0.73 | 0.116108 |
Target: 5'- cCCCAGUacCGCgACCAGCgaGGGCgCGGc -3' miRNA: 3'- -GGGUCA--GCG-UGGUUGgaCCCGgGCCc -5' |
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352 | 3' | -61.1 | AC_000011.1 | + | 6787 | 1.12 | 0.000116 |
Target: 5'- gCCCAGUCGCACCAACCUGGGCCCGGGg -3' miRNA: 3'- -GGGUCAGCGUGGUUGGACCCGGGCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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