Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3521 | 3' | -54.3 | NC_001623.1 | + | 87496 | 0.66 | 0.946679 |
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Target: 5'- -uGUUGGACGCCGugcgaucucUGUUGGAgaUGCCu -3' miRNA: 3'- ucCGACCUGUGGU---------GCAACUUgaGCGG- -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 38085 | 0.66 | 0.932308 |
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Target: 5'- cGGCcGG-CGCCAUuUUGGugUaCGCCa -3' miRNA: 3'- uCCGaCCuGUGGUGcAACUugA-GCGG- -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 2354 | 0.67 | 0.921495 |
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Target: 5'- uGGCuuuUGcGAUguuuGCCGCGUUGAACgCGCa -3' miRNA: 3'- uCCG---AC-CUG----UGGUGCAACUUGaGCGg -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 110119 | 0.67 | 0.920928 |
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Target: 5'- -uGCgaGGGCGCCGCGUUGGuccacacggccgaACU-GCCg -3' miRNA: 3'- ucCGa-CCUGUGGUGCAACU-------------UGAgCGG- -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 68563 | 0.69 | 0.836642 |
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Target: 5'- cAGuGCguaaaUGGGCGCCACGUggUGGGCgaaUGCCc -3' miRNA: 3'- -UC-CG-----ACCUGUGGUGCA--ACUUGa--GCGG- -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 82708 | 0.69 | 0.836642 |
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Target: 5'- -uGUUGGGCACCACGUUGc---CGCa -3' miRNA: 3'- ucCGACCUGUGGUGCAACuugaGCGg -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 87592 | 0.69 | 0.828169 |
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Target: 5'- aAGGCUgcgcGGGCGCgGCGgcGuugGCUCGCg -3' miRNA: 3'- -UCCGA----CCUGUGgUGCaaCu--UGAGCGg -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 108573 | 0.71 | 0.70449 |
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Target: 5'- aGGGCguugGGugGCCAacacGGACUCGUCg -3' miRNA: 3'- -UCCGa---CCugUGGUgcaaCUUGAGCGG- -5' |
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| 3521 | 3' | -54.3 | NC_001623.1 | + | 40516 | 1.13 | 0.002089 |
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Target: 5'- cAGGCUGGACACCACGUUGAACUCGCCg -3' miRNA: 3'- -UCCGACCUGUGGUGCAACUUGAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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