Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3521 | 5' | -58.4 | NC_001623.1 | + | 31081 | 0.66 | 0.777859 |
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Target: 5'- cCGGaCAAGgcuguccugcugUGuGCCCGUCGCGCGUa -3' miRNA: 3'- cGCC-GUUCa-----------ACcUGGGCGGCGCGUAg -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 64151 | 0.66 | 0.77042 |
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Target: 5'- gGCGuCGAGaUGGAaCgGCCGCGCAg- -3' miRNA: 3'- -CGCcGUUCaACCUgGgCGGCGCGUag -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 37338 | 0.67 | 0.741869 |
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Target: 5'- -gGGCGcuuGUUGGugCgGCgGCGgAUCg -3' miRNA: 3'- cgCCGUu--CAACCugGgCGgCGCgUAG- -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 87671 | 0.67 | 0.702515 |
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Target: 5'- cGCGGCGAcgacguccgcGUUu--CCCGCCGCGUAc- -3' miRNA: 3'- -CGCCGUU----------CAAccuGGGCGGCGCGUag -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 122332 | 0.67 | 0.692507 |
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Target: 5'- gGCGGCGAGUUGGcgGCaggCGCUG-GCAa- -3' miRNA: 3'- -CGCCGUUCAACC--UGg--GCGGCgCGUag -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 45998 | 0.69 | 0.631717 |
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Target: 5'- gGCGGCGucccgccgGGacaugucauguGCCCGCCGCGUgagAUCa -3' miRNA: 3'- -CGCCGUucaa----CC-----------UGGGCGGCGCG---UAG- -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 87834 | 0.69 | 0.58098 |
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Target: 5'- cGCGGCGGGaaacgcGGACgucgUCGCCGCGCcagcGUCc -3' miRNA: 3'- -CGCCGUUCaa----CCUG----GGCGGCGCG----UAG- -5' |
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| 3521 | 5' | -58.4 | NC_001623.1 | + | 40550 | 1.1 | 0.001268 |
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Target: 5'- uGCGGCAAGUUGGACCCGCCGCGCAUCc -3' miRNA: 3'- -CGCCGUUCAACCUGGGCGGCGCGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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