Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3522 | 3' | -49.7 | NC_001623.1 | + | 125112 | 0.66 | 0.996028 |
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Target: 5'- cUAGUCGUaGCGCCAGUaguGGGAUCa- -3' miRNA: 3'- aAUUAGCG-CGCGGUUAgu-CUUUGGcc -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 79414 | 0.67 | 0.992663 |
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Target: 5'- -cGAUCGUGCuGUacuuguGUCAGgcGCCGGc -3' miRNA: 3'- aaUUAGCGCG-CGgu----UAGUCuuUGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 116142 | 0.67 | 0.988887 |
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Target: 5'- --uAUUGC-CGCCAAaaAGAAAUCGGa -3' miRNA: 3'- aauUAGCGcGCGGUUagUCUUUGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 102006 | 0.67 | 0.988887 |
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Target: 5'- -gAGUCGCGCGucacgauauCCAcgCGGAGACgCGc -3' miRNA: 3'- aaUUAGCGCGC---------GGUuaGUCUUUG-GCc -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 37376 | 0.68 | 0.985629 |
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Target: 5'- gUGAUUGUGaaaugguaCCAAUCGGcGACCGGg -3' miRNA: 3'- aAUUAGCGCgc------GGUUAGUCuUUGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 6154 | 0.68 | 0.983748 |
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Target: 5'- ---uUCGgGCGCCGuuuUUGGuuuGACCGGu -3' miRNA: 3'- aauuAGCgCGCGGUu--AGUCu--UUGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 18481 | 0.68 | 0.976984 |
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Target: 5'- --cGUUGCGCGauaAAgggUAGGAGCCGGg -3' miRNA: 3'- aauUAGCGCGCgg-UUa--GUCUUUGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 113321 | 0.7 | 0.944658 |
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Target: 5'- --cGUCGCGCGgCA--CAGAAACCa- -3' miRNA: 3'- aauUAGCGCGCgGUuaGUCUUUGGcc -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 1255 | 0.72 | 0.898458 |
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Target: 5'- --cGUCGCGCGUCAcgCAcAAcACCGGa -3' miRNA: 3'- aauUAGCGCGCGGUuaGUcUU-UGGCC- -5' |
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| 3522 | 3' | -49.7 | NC_001623.1 | + | 46108 | 1.08 | 0.011075 |
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Target: 5'- gUUAAUCGCGCGCCAAUCAGAAACCGGc -3' miRNA: 3'- -AAUUAGCGCGCGGUUAGUCUUUGGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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