Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3522 | 5' | -43.2 | NC_001623.1 | + | 62847 | 0.66 | 1 |
Target: 5'- aGUG-GUcgAUGCGA--ACGUGGACGa -3' miRNA: 3'- aCGCuCAaaUGCGCUuuUGCAUUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 32577 | 0.66 | 1 |
Target: 5'- ---aAGUUUGCGUuuGAAAACGUcGACAa -3' miRNA: 3'- acgcUCAAAUGCG--CUUUUGCAuUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 53736 | 0.66 | 1 |
Target: 5'- gUGCuuGUuuguccaauuuaauuUUAUGCGAAGACGgcAACGu -3' miRNA: 3'- -ACGcuCA---------------AAUGCGCUUUUGCauUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 9854 | 0.66 | 1 |
Target: 5'- aGCGAGUUUgAUGCGGu--CGUcGAUg -3' miRNA: 3'- aCGCUCAAA-UGCGCUuuuGCAuUUGu -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 74114 | 0.67 | 0.999998 |
Target: 5'- uUGCaAGUagACGCG-AAACGUGcAACAu -3' miRNA: 3'- -ACGcUCAaaUGCGCuUUUGCAU-UUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 16133 | 0.68 | 0.999993 |
Target: 5'- uUGCGgaacAGcUUGgGCGAAAACGUcgGCGu -3' miRNA: 3'- -ACGC----UCaAAUgCGCUUUUGCAuuUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 76126 | 0.68 | 0.999993 |
Target: 5'- cGCGGGUgu-CGCGAGAcgaauuGCGUuucuuuGACGu -3' miRNA: 3'- aCGCUCAaauGCGCUUU------UGCAu-----UUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 67474 | 0.68 | 0.999986 |
Target: 5'- aGUGcGUUUGgGCGAcGACGUGuguACAg -3' miRNA: 3'- aCGCuCAAAUgCGCUuUUGCAUu--UGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 19708 | 0.69 | 0.99998 |
Target: 5'- gUGCGAgauugaaauGUUUAUgggucgauauaGCGAcGACGUGGACAa -3' miRNA: 3'- -ACGCU---------CAAAUG-----------CGCUuUUGCAUUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 2454 | 0.69 | 0.999972 |
Target: 5'- aGCGAGUcaACGCGGGcggggaguUGUAGGCGu -3' miRNA: 3'- aCGCUCAaaUGCGCUUuu------GCAUUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 6041 | 0.7 | 0.999837 |
Target: 5'- gGCGGGgcugGCgGCGGAGGCGgaGGCGg -3' miRNA: 3'- aCGCUCaaa-UG-CGCUUUUGCauUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 101146 | 0.71 | 0.999554 |
Target: 5'- gUGCGuGUgcgACGaCGGGuACGUGAGCGa -3' miRNA: 3'- -ACGCuCAaa-UGC-GCUUuUGCAUUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 37643 | 0.74 | 0.994339 |
Target: 5'- gGUGGGUUUgACGagagccauCGAAAACGUGGGCAg -3' miRNA: 3'- aCGCUCAAA-UGC--------GCUUUUGCAUUUGU- -5' |
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3522 | 5' | -43.2 | NC_001623.1 | + | 46143 | 1.11 | 0.037232 |
Target: 5'- cUGCGAGUUUACGCGAAAACGUAAACAa -3' miRNA: 3'- -ACGCUCAAAUGCGCUUUUGCAUUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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