Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3525 | 3' | -49.1 | NC_001623.1 | + | 65035 | 0.66 | 0.998374 |
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Target: 5'- aUAAUUCCc--GCCGAcaCGUUGCUCg -3' miRNA: 3'- aGUUAAGGaauUGGCUcaGCAGCGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 95646 | 0.66 | 0.997662 |
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Target: 5'- gUCAAUguac-AACCG-GUCGUCGUCUg -3' miRNA: 3'- -AGUUAaggaaUUGGCuCAGCAGCGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 65511 | 0.67 | 0.996652 |
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Target: 5'- -----aCCUUGuugguguACaGAGUCGUCGCCa -3' miRNA: 3'- aguuaaGGAAU-------UGgCUCAGCAGCGGg -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 121658 | 0.68 | 0.992861 |
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Target: 5'- cUCAAUUCUUUAACUuccGUCGUgUGCUCu -3' miRNA: 3'- -AGUUAAGGAAUUGGcu-CAGCA-GCGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 94480 | 0.68 | 0.992861 |
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Target: 5'- aUCGGUUUCUauuuacaaauUGGCCGAGcUGaUCGCCUu -3' miRNA: 3'- -AGUUAAGGA----------AUUGGCUCaGC-AGCGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 19456 | 0.7 | 0.975143 |
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Target: 5'- uUCAuUUCCgUUAGCagCGuGUCGUCGUCUa -3' miRNA: 3'- -AGUuAAGG-AAUUG--GCuCAGCAGCGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 80153 | 0.71 | 0.946464 |
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Target: 5'- cUAAUUCC---ACCGAGUCGcugcUCuGCCCu -3' miRNA: 3'- aGUUAAGGaauUGGCUCAGC----AG-CGGG- -5' |
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| 3525 | 3' | -49.1 | NC_001623.1 | + | 79960 | 1.15 | 0.00594 |
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Target: 5'- uUCAAUUCCUUAACCGAGUCGUCGCCCa -3' miRNA: 3'- -AGUUAAGGAAUUGGCUCAGCAGCGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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