Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3529 | 3' | -56 | NC_001623.1 | + | 57786 | 0.66 | 0.825834 |
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Target: 5'- cGUUUGGuugaacGCUGGCCGCUGUuguucgCGCCAAc -3' miRNA: 3'- -UAGACCu-----UGAUUGGCGGCG------GCGGUUu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 108218 | 0.66 | 0.817007 |
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Target: 5'- -aCUGGu----GCCGaCGCCGCCAAAc -3' miRNA: 3'- uaGACCuugauUGGCgGCGGCGGUUU- -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 129220 | 0.68 | 0.760621 |
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Target: 5'- aAUCUGGGucgGCggacgGAUCGCUGCCGCg--- -3' miRNA: 3'- -UAGACCU---UGa----UUGGCGGCGGCGguuu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 111819 | 0.68 | 0.714332 |
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Target: 5'- uGUCUGucGGCUucguuaaaauugccACCGCCGCCGCCGc- -3' miRNA: 3'- -UAGACc-UUGAu-------------UGGCGGCGGCGGUuu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 31042 | 0.7 | 0.615851 |
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Target: 5'- ---cGGAGCgcgAACgCGcCCGCCGCCGAc -3' miRNA: 3'- uagaCCUUGa--UUG-GC-GGCGGCGGUUu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 65910 | 0.72 | 0.482555 |
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Target: 5'- --gUGGuAgUAAUCGCCGCCGCCGGc -3' miRNA: 3'- uagACCuUgAUUGGCGGCGGCGGUUu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 29310 | 0.73 | 0.443299 |
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Target: 5'- -gCUGGAACUGAgcuuuuccuuuucCUGCUGCCGCUAAu -3' miRNA: 3'- uaGACCUUGAUU-------------GGCGGCGGCGGUUu -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 109558 | 0.75 | 0.340516 |
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Target: 5'- ----aGAAUgcGCCGCCGCCGCCAAAa -3' miRNA: 3'- uagacCUUGauUGGCGGCGGCGGUUU- -5' |
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| 3529 | 3' | -56 | NC_001623.1 | + | 109898 | 1.04 | 0.004095 |
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Target: 5'- uAUCUGGAACUAACCGCCGCCGCCAAAg -3' miRNA: 3'- -UAGACCUUGAUUGGCGGCGGCGGUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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