Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 353 | 3' | -64.2 | AC_000011.1 | + | 12272 | 0.66 | 0.234646 |
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Target: 5'- -aGGACCU-UCUCgUGCGUGGGgUUGg -3' miRNA: 3'- gaCCUGGAcGGAGgGCGCGCCCgAGC- -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 9297 | 0.66 | 0.223009 |
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Target: 5'- -gGGGCCUGCgUCgCCG-GCGGcGCaCGg -3' miRNA: 3'- gaCCUGGACGgAG-GGCgCGCC-CGaGC- -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 17830 | 0.67 | 0.191014 |
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Target: 5'- -aGGAgCUGCCUCCCcuggacaaGCG-GGGCgagaagCGa -3' miRNA: 3'- gaCCUgGACGGAGGG--------CGCgCCCGa-----GC- -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 22711 | 0.67 | 0.18609 |
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Target: 5'- aUGGGCC-GCUUCCUGCa-GGGCUUc -3' miRNA: 3'- gACCUGGaCGGAGGGCGcgCCCGAGc -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 16550 | 0.67 | 0.176578 |
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Target: 5'- -aGGugCUGCCgaccgcggCgCCGCGCcggGGGUUCa -3' miRNA: 3'- gaCCugGACGGa-------G-GGCGCG---CCCGAGc -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 20249 | 0.67 | 0.171985 |
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Target: 5'- aCUGGGCC-GCCUUCCGCgGCuGGUccuUCa -3' miRNA: 3'- -GACCUGGaCGGAGGGCG-CGcCCG---AGc -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 17780 | 0.68 | 0.1675 |
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Target: 5'- cCUGGACCcggUGCCgCCCGC-C-GGCUCc -3' miRNA: 3'- -GACCUGG---ACGGaGGGCGcGcCCGAGc -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 15906 | 0.68 | 0.146614 |
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Target: 5'- aUGGcCCUGCgUCCCGUGCGccuGGCccugCGc -3' miRNA: 3'- gACCuGGACGgAGGGCGCGC---CCGa---GC- -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 6332 | 0.69 | 0.128129 |
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Target: 5'- aCUGGugGCCa-CCUCgCCGCGCagGGGCUCa -3' miRNA: 3'- -GACC--UGGacGGAG-GGCGCG--CCCGAGc -5' |
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| 353 | 3' | -64.2 | AC_000011.1 | + | 8203 | 1.07 | 0.000118 |
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Target: 5'- uCUGGACCUGCCUCCCGCGCGGGCUCGu -3' miRNA: 3'- -GACCUGGACGGAGGGCGCGCCCGAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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