Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3530 | 3' | -50.2 | NC_001623.1 | + | 36431 | 0.67 | 0.980752 |
Target: 5'- --aACAAGAguuauaugAAGCaAUUGCUGCgCGAGg -3' miRNA: 3'- uuaUGUUCU--------UUCGgUAGCGACG-GCUC- -5' |
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3530 | 3' | -50.2 | NC_001623.1 | + | 10699 | 0.69 | 0.936935 |
Target: 5'- --gACAAcGAcGCCAUCGCcGCCGAa -3' miRNA: 3'- uuaUGUUcUUuCGGUAGCGaCGGCUc -5' |
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3530 | 3' | -50.2 | NC_001623.1 | + | 113444 | 0.71 | 0.893932 |
Target: 5'- uGUGCGuuuugaauGAAAGCCGUCGCUuGUCGGc -3' miRNA: 3'- uUAUGUu-------CUUUCGGUAGCGA-CGGCUc -5' |
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3530 | 3' | -50.2 | NC_001623.1 | + | 94138 | 0.76 | 0.633681 |
Target: 5'- aGAUGCAAGAccGCCAUCGCUuuugGCCa-- -3' miRNA: 3'- -UUAUGUUCUuuCGGUAGCGA----CGGcuc -5' |
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3530 | 3' | -50.2 | NC_001623.1 | + | 124034 | 0.76 | 0.655571 |
Target: 5'- cGUACAGGAAAGCCAUCaucUUGCgGGGa -3' miRNA: 3'- uUAUGUUCUUUCGGUAGc--GACGgCUC- -5' |
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3530 | 3' | -50.2 | NC_001623.1 | + | 111860 | 1.06 | 0.011141 |
Target: 5'- cAAUACAAGAAAGCCAUCGCUGCCGAGc -3' miRNA: 3'- -UUAUGUUCUUUCGGUAGCGACGGCUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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