Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
356 | 3' | -62.7 | AC_000011.1 | + | 3825 | 0.66 | 0.27443 |
Target: 5'- uGACCcaGCGCCU-GGGCGa-GCUGACCc -3' miRNA: 3'- -CUGG--UGCGGAcCCCGUacCGGCUGGc -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 9867 | 0.66 | 0.235772 |
Target: 5'- aGCCGCG-CUGGGGC-UGGaCGAgCGc -3' miRNA: 3'- cUGGUGCgGACCCCGuACCgGCUgGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 24799 | 0.67 | 0.218215 |
Target: 5'- cACCAcCGCCUcGGaCcUGGCCGACCu -3' miRNA: 3'- cUGGU-GCGGAcCCcGuACCGGCUGGc -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 8466 | 0.67 | 0.218215 |
Target: 5'- gGACgCGCGCCgGGcGGCAgGGgCGGCuCGg -3' miRNA: 3'- -CUG-GUGCGGaCC-CCGUaCCgGCUG-GC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 24677 | 0.67 | 0.212616 |
Target: 5'- uGCCACaCCUGgcagacGGGCAUGGgCGugUGg -3' miRNA: 3'- cUGGUGcGGAC------CCCGUACCgGCugGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 10989 | 0.68 | 0.186445 |
Target: 5'- gGGCUggcGCGCCUgGGGGCGUcGUCG-CCGg -3' miRNA: 3'- -CUGG---UGCGGA-CCCCGUAcCGGCuGGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 13621 | 0.68 | 0.186445 |
Target: 5'- gGGCCACgaGCC-GGGGCAgcGCCGcCCGu -3' miRNA: 3'- -CUGGUG--CGGaCCCCGUacCGGCuGGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 23529 | 0.69 | 0.158824 |
Target: 5'- cGGCCGCGUCggaGGuGGCggGGCUGGgCGg -3' miRNA: 3'- -CUGGUGCGGa--CC-CCGuaCCGGCUgGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 12527 | 0.71 | 0.108249 |
Target: 5'- -uCCugGCCccGGGGCAcguUGGCgGGCUGg -3' miRNA: 3'- cuGGugCGGa-CCCCGU---ACCGgCUGGC- -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 21259 | 0.73 | 0.081827 |
Target: 5'- cGGCCGCGagaCCgGGGGCGagcacUGGCUGGCCu -3' miRNA: 3'- -CUGGUGC---GGaCCCCGU-----ACCGGCUGGc -5' |
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356 | 3' | -62.7 | AC_000011.1 | + | 9807 | 1.07 | 0.000153 |
Target: 5'- gGACCACGCCUGGGGCAUGGCCGACCGu -3' miRNA: 3'- -CUGGUGCGGACCCCGUACCGGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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