Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
356 | 5' | -58.1 | AC_000011.1 | + | 21225 | 0.66 | 0.434635 |
Target: 5'- cCCAGgAAGuaGGGCCCGcAGcCCa- -3' miRNA: 3'- aGGUCgUUCggCCCGGGUuUCuGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 12228 | 0.66 | 0.415165 |
Target: 5'- cUCCAGgauGGCCGaGaGCCgGuuGGCCUGg -3' miRNA: 3'- -AGGUCgu-UCGGC-C-CGGgUuuCUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 12851 | 0.66 | 0.405633 |
Target: 5'- cCCAcGUGAGCCGcGCCCu-GGGCCa- -3' miRNA: 3'- aGGU-CGUUCGGCcCGGGuuUCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 7566 | 0.66 | 0.396239 |
Target: 5'- cUCgAGC-AGCaCGGGCUCAgcGACCc- -3' miRNA: 3'- -AGgUCGuUCG-GCCCGGGUuuCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 11493 | 0.66 | 0.396239 |
Target: 5'- aCCgAGCccgaGGGCCGcuGGCUCcuGGACCUGg -3' miRNA: 3'- aGG-UCG----UUCGGC--CCGGGuuUCUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 8479 | 0.66 | 0.386987 |
Target: 5'- -gCGGCAggggcGGCUcgGGGCCCGGAGGCa-- -3' miRNA: 3'- agGUCGU-----UCGG--CCCGGGUUUCUGgac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 21178 | 0.66 | 0.386987 |
Target: 5'- cUCCuGCucGCCGGaGCCCGc-GGCCa- -3' miRNA: 3'- -AGGuCGuuCGGCC-CGGGUuuCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 8324 | 0.66 | 0.384239 |
Target: 5'- cUCCuGCAAgucaaccgcgcgccGCCGcuGCCCAcuGACCUGa -3' miRNA: 3'- -AGGuCGUU--------------CGGCc-CGGGUuuCUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 6270 | 0.67 | 0.360098 |
Target: 5'- gUCAGCucGUCGGGCaCGAuucuGACCUGc -3' miRNA: 3'- aGGUCGuuCGGCCCGgGUUu---CUGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 11406 | 0.67 | 0.342911 |
Target: 5'- aCCAGCAAGCCGcugacGGCgCAGcuguuCCUGg -3' miRNA: 3'- aGGUCGUUCGGC-----CCGgGUUucu--GGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 26514 | 0.67 | 0.334541 |
Target: 5'- cUCAGC--GCCGGGCCCGcgauGAUCUc -3' miRNA: 3'- aGGUCGuuCGGCCCGGGUuu--CUGGAc -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 16401 | 0.68 | 0.326322 |
Target: 5'- gCCggAGCGcucGCCGGGCgUGAAGACCa- -3' miRNA: 3'- aGG--UCGUu--CGGCCCGgGUUUCUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 5151 | 0.68 | 0.310337 |
Target: 5'- gUCCAGCGcAGCCaGGGUCCGGu--CCUu -3' miRNA: 3'- -AGGUCGU-UCGG-CCCGGGUUucuGGAc -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 17839 | 0.68 | 0.310336 |
Target: 5'- gCCGGCGGGCggcacCGGGUCCAGGcGGCUg- -3' miRNA: 3'- aGGUCGUUCG-----GCCCGGGUUU-CUGGac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 8206 | 0.68 | 0.294954 |
Target: 5'- ---cGCAGGCCGGagcuGUCCAGGGuCCUGa -3' miRNA: 3'- agguCGUUCGGCC----CGGGUUUCuGGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 11423 | 0.69 | 0.290456 |
Target: 5'- cUCCAGCAGGUCccacaggugcaucaGGCCCAGGGucACCUc -3' miRNA: 3'- -AGGUCGUUCGGc-------------CCGGGUUUC--UGGAc -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 11895 | 0.69 | 0.287488 |
Target: 5'- cCCAGCc-GCCGGGCCUuGGAGGCg-- -3' miRNA: 3'- aGGUCGuuCGGCCCGGG-UUUCUGgac -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 9433 | 0.69 | 0.28675 |
Target: 5'- cUCCAGguGGCCGGGgggguCCCcguugggcagggaGAGGGCgCUGa -3' miRNA: 3'- -AGGUCguUCGGCCC-----GGG-------------UUUCUG-GAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 26516 | 0.71 | 0.182469 |
Target: 5'- aCCGGC-GGCCaGGCCCAucuGGGgCUGg -3' miRNA: 3'- aGGUCGuUCGGcCCGGGUu--UCUgGAC- -5' |
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356 | 5' | -58.1 | AC_000011.1 | + | 21127 | 0.71 | 0.182469 |
Target: 5'- cUCCGGCGAGCaGGaGCUCAGGgccaucauccgcGACCUGg -3' miRNA: 3'- -AGGUCGUUCGgCC-CGGGUUU------------CUGGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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