Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3576 | 3' | -47.6 | NC_001650.1 | + | 4279 | 0.66 | 0.99993 |
Target: 5'- gCCUGGcagggcCUcUCCAUAUUUGGcAGGUu -3' miRNA: 3'- gGGACCu-----GAaAGGUAUAGACCuUUCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 170986 | 0.66 | 0.99993 |
Target: 5'- gCCUGGcagggcCUcUCCAUAUUUGGcAGGUu -3' miRNA: 3'- gGGACCu-----GAaAGGUAUAGACCuUUCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 86307 | 0.66 | 0.999907 |
Target: 5'- cUCCUcggcGGACUggUUCCAguaCUGGGAGGc -3' miRNA: 3'- -GGGA----CCUGA--AAGGUauaGACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 2231 | 0.66 | 0.999907 |
Target: 5'- cCCCUGGcC--UCCAUGgccaaUGGGGAGa -3' miRNA: 3'- -GGGACCuGaaAGGUAUag---ACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 168938 | 0.66 | 0.999907 |
Target: 5'- cCCCUGGcC--UCCAUGgccaaUGGGGAGa -3' miRNA: 3'- -GGGACCuGaaAGGUAUag---ACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 137239 | 0.66 | 0.999879 |
Target: 5'- gCCCaUGGACcugcacUCCcaGUUUGGGAGGUg -3' miRNA: 3'- -GGG-ACCUGaa----AGGuaUAGACCUUUCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 145773 | 0.67 | 0.999674 |
Target: 5'- gCCCggGGACUUcaUCCAcGUcCUGGggGa- -3' miRNA: 3'- -GGGa-CCUGAA--AGGUaUA-GACCuuUca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 71342 | 0.68 | 0.999347 |
Target: 5'- aCCUGGGCc-UCCGUGuacauguUCUGGAGGc- -3' miRNA: 3'- gGGACCUGaaAGGUAU-------AGACCUUUca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 145325 | 0.68 | 0.999031 |
Target: 5'- cCCUUGGAaaauaacCCAUgaucuGUCUGGAAGGg -3' miRNA: 3'- -GGGACCUgaaa---GGUA-----UAGACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 139840 | 0.69 | 0.997922 |
Target: 5'- cCCCUGGAUUUUgCAccucgcGUCUGGAc--- -3' miRNA: 3'- -GGGACCUGAAAgGUa-----UAGACCUuuca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 38222 | 0.69 | 0.997052 |
Target: 5'- aCgCUGGACgccgUUUgGUAUCgagGGGAGGUa -3' miRNA: 3'- -GgGACCUGa---AAGgUAUAGa--CCUUUCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 98626 | 0.69 | 0.996515 |
Target: 5'- cCCCUGcagcgucaACUgUCCGUcUCUGGGAAGUu -3' miRNA: 3'- -GGGACc-------UGAaAGGUAuAGACCUUUCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 101978 | 0.69 | 0.996515 |
Target: 5'- aCCUGGGCUcggggUCCcugAUCUGGAc--- -3' miRNA: 3'- gGGACCUGAa----AGGua-UAGACCUuuca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 115028 | 0.69 | 0.996515 |
Target: 5'- cCCUUGGACUUUugguccuugagCCAcuUCUGGAGcauGGUg -3' miRNA: 3'- -GGGACCUGAAA-----------GGUauAGACCUU---UCA- -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 145264 | 0.7 | 0.993116 |
Target: 5'- cCCCUGGAaaaaaacaucCCAUgaucuGUCUGGAGGGg -3' miRNA: 3'- -GGGACCUgaaa------GGUA-----UAGACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 110397 | 0.72 | 0.985435 |
Target: 5'- aCUUGGACUUcUCCGaAUCUGaGGAGGa -3' miRNA: 3'- gGGACCUGAA-AGGUaUAGAC-CUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 158860 | 0.72 | 0.983554 |
Target: 5'- aCCUGGACcc-CCGUAgccgcCUGGAGGGa -3' miRNA: 3'- gGGACCUGaaaGGUAUa----GACCUUUCa -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 140421 | 0.75 | 0.939958 |
Target: 5'- uCCCUauagGGugUgUCCAUAUCUGGAGu-- -3' miRNA: 3'- -GGGA----CCugAaAGGUAUAGACCUUuca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 54243 | 0.75 | 0.929597 |
Target: 5'- cCCCUauagGGugUgUCCAUAUCUGGAGu-- -3' miRNA: 3'- -GGGA----CCugAaAGGUAUAGACCUUuca -5' |
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3576 | 3' | -47.6 | NC_001650.1 | + | 4354 | 1.1 | 0.019681 |
Target: 5'- cCCCUGGACUUUCCAUAUCUGGAAAGUu -3' miRNA: 3'- -GGGACCUGAAAGGUAUAGACCUUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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