Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3578 | 3' | -51.8 | NC_001650.1 | + | 175532 | 0.67 | 0.98399 |
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Target: 5'- uGGG-GGUCUgguggguguGGGGGUCcUUGGGAu -3' miRNA: 3'- uCCCaCCAGGau-------CUCCCAGaAAUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 179033 | 0.66 | 0.987427 |
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Target: 5'- uGGGUGuGUgCCaGGGGGGUUUggggUGGGGc -3' miRNA: 3'- uCCCAC-CA-GGaUCUCCCAGAa---AUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 179228 | 0.7 | 0.918561 |
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Target: 5'- uGGGUGGUCCcuucucucuuguacaUGG-GGGUCccUGGGAg -3' miRNA: 3'- uCCCACCAGG---------------AUCuCCCAGaaAUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 180848 | 0.66 | 0.992574 |
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Target: 5'- gGGGGcuccaUGGUCCc-GGGGGUCcagGGGGg -3' miRNA: 3'- -UCCC-----ACCAGGauCUCCCAGaaaUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 181036 | 0.67 | 0.98399 |
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Target: 5'- cGGGUGGUCa---GGGGUCcuggccugUAAGAg -3' miRNA: 3'- uCCCACCAGgaucUCCCAGaa------AUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 181407 | 0.71 | 0.890655 |
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Target: 5'- cGGG-GGUCCaAGGGGGUUggcaGAGAu -3' miRNA: 3'- uCCCaCCAGGaUCUCCCAGaaa-UUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 181594 | 0.66 | 0.991481 |
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Target: 5'- cGGGUccgGGUCCgcgaGGAGGGggg--GAGAg -3' miRNA: 3'- uCCCA---CCAGGa---UCUCCCagaaaUUCU- -5' |
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| 3578 | 3' | -51.8 | NC_001650.1 | + | 183225 | 0.67 | 0.979883 |
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Target: 5'- uGGGUGG-CCacAGAGGGUCa------ -3' miRNA: 3'- uCCCACCaGGa-UCUCCCAGaaauucu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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