Results 1 - 18 of 18 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
358 | 5' | -59.3 | AC_000011.1 | + | 10166 | 0.66 | 0.412571 |
Target: 5'- gCGGCcaucgCUCGG-UGGCGGgGGCgCCGg -3' miRNA: 3'- gGUCGaa---GAGCCuGUCGCCgUCG-GGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 8459 | 0.66 | 0.39412 |
Target: 5'- gCGGCgaggacgCgcgcCGGGCGGCaggGGCGGCUCGg -3' miRNA: 3'- gGUCGaa-----Ga---GCCUGUCG---CCGUCGGGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 17855 | 0.66 | 0.385096 |
Target: 5'- gCGGCaUCUccaCGGAgcCGGCgGGCGGCaCCGg -3' miRNA: 3'- gGUCGaAGA---GCCU--GUCG-CCGUCG-GGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 21902 | 0.66 | 0.37621 |
Target: 5'- aUCAGCagUUUGGGCAGCGgGguGUCgGg -3' miRNA: 3'- -GGUCGaaGAGCCUGUCGC-CguCGGgC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 26731 | 0.66 | 0.37621 |
Target: 5'- uCCAGCUgg-CGGGCGGCGcCA-CCCu -3' miRNA: 3'- -GGUCGAagaGCCUGUCGCcGUcGGGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 13694 | 0.67 | 0.333898 |
Target: 5'- aCCGGCgUUUaCGGGCGGCG-CuGCCCc -3' miRNA: 3'- -GGUCGaAGA-GCCUGUCGCcGuCGGGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 11895 | 0.67 | 0.325864 |
Target: 5'- cCCAGCcgccgggcCUUGGagGCGGCGGCAGgaCCCu -3' miRNA: 3'- -GGUCGaa------GAGCC--UGUCGCCGUC--GGGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 2941 | 0.67 | 0.317195 |
Target: 5'- gCAGCccgUCUCGGuagaggcGCAGUGGCGgauucuggcuucGCCCu -3' miRNA: 3'- gGUCGa--AGAGCC-------UGUCGCCGU------------CGGGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 1085 | 0.69 | 0.266786 |
Target: 5'- aCAGCcgugUC-CGGGCAGaggcgGGCAGUCCa -3' miRNA: 3'- gGUCGa---AGaGCCUGUCg----CCGUCGGGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 17979 | 0.69 | 0.260042 |
Target: 5'- cCCAGUUUCaccgccuccUCGuACGGgGGCGGCUCGu -3' miRNA: 3'- -GGUCGAAG---------AGCcUGUCgCCGUCGGGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 18304 | 0.69 | 0.246973 |
Target: 5'- uCCucCUUCUggUGGACAGCGGCGGCg-- -3' miRNA: 3'- -GGucGAAGA--GCCUGUCGCCGUCGggc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 8245 | 0.69 | 0.240644 |
Target: 5'- gUCAGg-UCagUGGGCAGCGGCGGCgCGc -3' miRNA: 3'- -GGUCgaAGa-GCCUGUCGCCGUCGgGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 17262 | 0.71 | 0.189616 |
Target: 5'- gCCAGcCUUCUaCGGcGCGGUuuucuuccucGGUAGCCCGu -3' miRNA: 3'- -GGUC-GAAGA-GCC-UGUCG----------CCGUCGGGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 12733 | 0.72 | 0.156745 |
Target: 5'- gCCccCUUCaCGGACAGCGGCAGCaucaaCCGc -3' miRNA: 3'- -GGucGAAGaGCCUGUCGCCGUCG-----GGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 13430 | 0.72 | 0.152493 |
Target: 5'- aCUGGCggcCUCGGGCAccgccGCGGCAGCaCCc -3' miRNA: 3'- -GGUCGaa-GAGCCUGU-----CGCCGUCG-GGc -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 9360 | 0.73 | 0.144301 |
Target: 5'- gCCGGCgucgcauggUCUCGGugA-CGGCGcGCCCGu -3' miRNA: 3'- -GGUCGa--------AGAGCCugUcGCCGU-CGGGC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 33883 | 0.74 | 0.112209 |
Target: 5'- gCAGCUUgaCGGAgCGGCGGCGGCagCGa -3' miRNA: 3'- gGUCGAAgaGCCU-GUCGCCGUCGg-GC- -5' |
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358 | 5' | -59.3 | AC_000011.1 | + | 11622 | 1.09 | 0.000235 |
Target: 5'- gCCAGCUUCUCGGACAGCGGCAGCCCGc -3' miRNA: 3'- -GGUCGAAGAGCCUGUCGCCGUCGGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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