miRNA: 3'- uaCGUACCCCCACAa--UC-GGUU-GGUUg -5'
miRNA: 3'- uaCGUACCCCC-ACaaUCGgUUGGUug -5'
miRNA: 3'- uaCGUACCCCCACaAUCGGuuGGUUG- -5'
miRNA: 3'- -UACGUACCCCCACAAUCGGUUGGUUG- -5'
miRNA: 3'- -UACGUACCCCCACAAUCGGUUGGUUG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3584 | 3' | -53.5 | NC_001650.1 | + | 175480 | 0.73 | 0.685257 |
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Target: 5'- -aGCGUGGGGGUGUgagGGaCCGAuUCAAa -3' miRNA: 3'- uaCGUACCCCCACAa--UC-GGUU-GGUUg -5' |
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| 3584 | 3' | -53.5 | NC_001650.1 | + | 133222 | 0.74 | 0.632816 |
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Target: 5'- -gGCGUGGGGGcUGggAGCgGGCCGc- -3' miRNA: 3'- uaCGUACCCCC-ACaaUCGgUUGGUug -5' |
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| 3584 | 3' | -53.5 | NC_001650.1 | + | 145407 | 0.86 | 0.133367 |
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Target: 5'- -cGCGUGGGGGUGgUAGCCcuCCAGCg -3' miRNA: 3'- uaCGUACCCCCACaAUCGGuuGGUUG- -5' |
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| 3584 | 3' | -53.5 | NC_001650.1 | + | 174560 | 1.07 | 0.006489 |
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Target: 5'- uAUGCAUGGGGGUGUUAGCCAACCAACc -3' miRNA: 3'- -UACGUACCCCCACAAUCGGUUGGUUG- -5' |
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| 3584 | 3' | -53.5 | NC_001650.1 | + | 7853 | 1.07 | 0.006489 |
|
Target: 5'- uAUGCAUGGGGGUGUUAGCCAACCAACc -3' miRNA: 3'- -UACGUACCCCCACAAUCGGUUGGUUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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