Results 1 - 20 of 20 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3587 | 3' | -51.2 | NC_001650.1 | + | 99635 | 0.66 | 0.995369 |
Target: 5'- gCACcucGGUGGUCCCGGuGGUGgGCUuGGc -3' miRNA: 3'- -GUGa--UCACCAGGGUU-UCACgUGGuUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 24359 | 0.66 | 0.995369 |
Target: 5'- gGCgUGGaGGUCCCAcucGGUGCGCauggaGAGg -3' miRNA: 3'- gUG-AUCaCCAGGGUu--UCACGUGg----UUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 120361 | 0.66 | 0.99461 |
Target: 5'- uGCUGGUGGaCCUGGAGUcCGCCc-- -3' miRNA: 3'- gUGAUCACCaGGGUUUCAcGUGGuuc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 41923 | 0.66 | 0.99461 |
Target: 5'- --gUGGUGGUCC---AGaGCACCGAGc -3' miRNA: 3'- gugAUCACCAGGguuUCaCGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 91671 | 0.66 | 0.991712 |
Target: 5'- aCACUGGcugaggugGGUCuCCAGgguGUGCACCu-- -3' miRNA: 3'- -GUGAUCa-------CCAG-GGUUu--CACGUGGuuc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 83662 | 0.66 | 0.991712 |
Target: 5'- gCGCaGGUGGUCCCucuGG-GcCACCAu- -3' miRNA: 3'- -GUGaUCACCAGGGuu-UCaC-GUGGUuc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 78517 | 0.67 | 0.987699 |
Target: 5'- aACg---GGcUCCUAuuuAAGUGCACCAAGa -3' miRNA: 3'- gUGaucaCC-AGGGU---UUCACGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 68623 | 0.67 | 0.984285 |
Target: 5'- aCACgu-UGaUCCCGAAcccGUGCACCGAGa -3' miRNA: 3'- -GUGaucACcAGGGUUU---CACGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 41744 | 0.68 | 0.980194 |
Target: 5'- gCugUGGUGGUCCaagacaccaaaCAGAGUGUugggaaGCCAGc -3' miRNA: 3'- -GugAUCACCAGG-----------GUUUCACG------UGGUUc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 102519 | 0.68 | 0.980194 |
Target: 5'- uGCUGGgcgGGUCCCAuaAAGUacagaGCGCCc-- -3' miRNA: 3'- gUGAUCa--CCAGGGU--UUCA-----CGUGGuuc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 26924 | 0.68 | 0.97997 |
Target: 5'- aGCUGGUGGUgguagagucccagUCCAGAgcGUGCGCguGGg -3' miRNA: 3'- gUGAUCACCA-------------GGGUUU--CACGUGguUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 36345 | 0.68 | 0.975358 |
Target: 5'- gGCcggGGUGGUgCCCAuAGacaUGUACCAGGu -3' miRNA: 3'- gUGa--UCACCA-GGGUuUC---ACGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 134540 | 0.69 | 0.963199 |
Target: 5'- aCACggAG-GGcCCCGGGGgGCGCCGGGg -3' miRNA: 3'- -GUGa-UCaCCaGGGUUUCaCGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 29198 | 0.69 | 0.96285 |
Target: 5'- aCACUGGggucacgGGcaacUCCCAGaucuuccAGUGCGCCAAc -3' miRNA: 3'- -GUGAUCa------CC----AGGGUU-------UCACGUGGUUc -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 141384 | 0.69 | 0.959601 |
Target: 5'- gGCcGG-GGUcaCCCAGAGUucGCGCCAGGg -3' miRNA: 3'- gUGaUCaCCA--GGGUUUCA--CGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 175734 | 0.71 | 0.89722 |
Target: 5'- uCACUugguAGUGGUCUCGAAG-GCACUcAGu -3' miRNA: 3'- -GUGA----UCACCAGGGUUUCaCGUGGuUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 9027 | 0.71 | 0.89722 |
Target: 5'- uCACUugguAGUGGUCUCGAAG-GCACUcAGu -3' miRNA: 3'- -GUGA----UCACCAGGGUUUCaCGUGGuUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 159339 | 0.72 | 0.886187 |
Target: 5'- uGCUGGUGGUgcugcucguacagagCCCAcccAGGaGCGCCAGGa -3' miRNA: 3'- gUGAUCACCA---------------GGGU---UUCaCGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 9280 | 1.1 | 0.008239 |
Target: 5'- cCACUAGUGGUCCCAAAGUGCACCAAGa -3' miRNA: 3'- -GUGAUCACCAGGGUUUCACGUGGUUC- -5' |
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3587 | 3' | -51.2 | NC_001650.1 | + | 175987 | 1.1 | 0.008239 |
Target: 5'- cCACUAGUGGUCCCAAAGUGCACCAAGa -3' miRNA: 3'- -GUGAUCACCAGGGUUUCACGUGGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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