Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3589 | 3' | -45.8 | NC_001650.1 | + | 57535 | 0.66 | 0.99999 |
Target: 5'- ---uGAGGCgccgGCUGGGgcugGGgcUGGGc -3' miRNA: 3'- uuuuCUCCGa---UGACCUaa--UCaaACCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 62131 | 0.67 | 0.99995 |
Target: 5'- cGGAGGAGGCUGuuccCUGGAgcacccagcaaccuaUGGggUGGGa -3' miRNA: 3'- -UUUUCUCCGAU----GACCUa--------------AUCaaACCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 12026 | 0.68 | 0.999662 |
Target: 5'- gGAGAGuGGCUAUUGGAUUAugcuagcaGGAg -3' miRNA: 3'- -UUUUCuCCGAUGACCUAAUcaaa----CCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 178733 | 0.68 | 0.999662 |
Target: 5'- gGAGAGuGGCUAUUGGAUUAugcuagcaGGAg -3' miRNA: 3'- -UUUUCuCCGAUGACCUAAUcaaa----CCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 167361 | 0.68 | 0.999662 |
Target: 5'- gGGGAGGGGCUugUGuGcgUGGgcgGGGg -3' miRNA: 3'- -UUUUCUCCGAugAC-CuaAUCaaaCCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 654 | 0.68 | 0.999662 |
Target: 5'- gGGGAGGGGCUugUGuGcgUGGgcgGGGg -3' miRNA: 3'- -UUUUCUCCGAugAC-CuaAUCaaaCCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 43483 | 0.69 | 0.999326 |
Target: 5'- gGGAGGGGGuUUACUGGAaaugGGUgagGGAg -3' miRNA: 3'- -UUUUCUCC-GAUGACCUaa--UCAaa-CCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 180315 | 0.69 | 0.999162 |
Target: 5'- aAAAAGAGGaguUAUUGGAUUAGUUUu-- -3' miRNA: 3'- -UUUUCUCCg--AUGACCUAAUCAAAccu -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 13608 | 0.69 | 0.999162 |
Target: 5'- aAAAAGAGGaguUAUUGGAUUAGUUUu-- -3' miRNA: 3'- -UUUUCUCCg--AUGACCUAAUCAAAccu -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 105121 | 0.7 | 0.998454 |
Target: 5'- cAAAGGAGGCUAUggUGGGUucagUGGUUucUGGu -3' miRNA: 3'- -UUUUCUCCGAUG--ACCUA----AUCAA--ACCu -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 62385 | 0.7 | 0.997742 |
Target: 5'- -cAGGGGGCUACUcuGUUGGUUggGGAg -3' miRNA: 3'- uuUUCUCCGAUGAccUAAUCAAa-CCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 178984 | 1.08 | 0.032532 |
Target: 5'- gAAAAGAGGCUACUGGAUUAGUUUGGAu -3' miRNA: 3'- -UUUUCUCCGAUGACCUAAUCAAACCU- -5' |
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3589 | 3' | -45.8 | NC_001650.1 | + | 12277 | 1.08 | 0.032532 |
Target: 5'- gAAAAGAGGCUACUGGAUUAGUUUGGAu -3' miRNA: 3'- -UUUUCUCCGAUGACCUAAUCAAACCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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