Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
359 | 3' | -62.9 | AC_000011.1 | + | 3669 | 0.66 | 0.249383 |
Target: 5'- cCGCCGCagCUGCUGcaucugcCGCcagCGCCGUGCGc -3' miRNA: 3'- -GCGGCG--GACGACc------GCGa--GUGGCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 15618 | 0.66 | 0.249383 |
Target: 5'- gGCCGCgUGC-GGuCGCgcacCACCGUcgACGa -3' miRNA: 3'- gCGGCGgACGaCC-GCGa---GUGGCG--UGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 16320 | 0.67 | 0.213743 |
Target: 5'- gCGCCGCCgGggGGCGaaCUCG-CGCACa -3' miRNA: 3'- -GCGGCGGaCgaCCGC--GAGUgGCGUGc -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 15687 | 0.67 | 0.208247 |
Target: 5'- aCGUCGUCgacGgUGGUGCgcgACCGCACGc -3' miRNA: 3'- -GCGGCGGa--CgACCGCGag-UGGCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 24155 | 0.67 | 0.202872 |
Target: 5'- gCGCgGCUgGC-GGUGCUCAaaCGCACGg -3' miRNA: 3'- -GCGgCGGaCGaCCGCGAGUg-GCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 17042 | 0.67 | 0.202872 |
Target: 5'- uGCCGCC--CUGGUGCggagagugUACCGcCGCGg -3' miRNA: 3'- gCGGCGGacGACCGCGa-------GUGGC-GUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 28403 | 0.67 | 0.197619 |
Target: 5'- uCGCUuacaGCCUGUgcacGGCGCuaaUCACCGCuauCGu -3' miRNA: 3'- -GCGG----CGGACGa---CCGCG---AGUGGCGu--GC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 11455 | 0.67 | 0.197619 |
Target: 5'- aGCgGCUUGCUGGUGggguuCUGCACGa -3' miRNA: 3'- gCGgCGGACGACCGCgagu-GGCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 8529 | 0.68 | 0.182566 |
Target: 5'- aGCCGCCccUGCcgcccGGCGCgcguccUCGCCGC-CGc -3' miRNA: 3'- gCGGCGG--ACGa----CCGCG------AGUGGCGuGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 13975 | 0.68 | 0.173103 |
Target: 5'- cCGCCaucGCCUGCUGcaucaCGCUC-UCGUACGa -3' miRNA: 3'- -GCGG---CGGACGACc----GCGAGuGGCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 15863 | 0.68 | 0.173103 |
Target: 5'- gCGCCGCgCgcaugGCgggGGUGCUCcgguGCCGC-CGg -3' miRNA: 3'- -GCGGCG-Ga----CGa--CCGCGAG----UGGCGuGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 21619 | 0.69 | 0.159727 |
Target: 5'- gCGCCGCaaccaGgaGGCGCUCuACCGCu-- -3' miRNA: 3'- -GCGGCGga---CgaCCGCGAG-UGGCGugc -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 23892 | 0.69 | 0.147292 |
Target: 5'- uCGCagguGCCauuggGCUGGCGCUUgggggGCaCGCGCGg -3' miRNA: 3'- -GCGg---CGGa----CGACCGCGAG-----UG-GCGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 33978 | 0.69 | 0.143347 |
Target: 5'- cCGCaUGCgCUGCU-GCGCcCGCCGCACc -3' miRNA: 3'- -GCG-GCG-GACGAcCGCGaGUGGCGUGc -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 10994 | 0.7 | 0.132084 |
Target: 5'- gGCgCGCCUGggGGCGUcgUCGCCGgaGCGg -3' miRNA: 3'- gCG-GCGGACgaCCGCG--AGUGGCg-UGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 17318 | 0.7 | 0.132084 |
Target: 5'- -aCCGCUUGCugaUGGCGCgcCGCCGC-CGg -3' miRNA: 3'- gcGGCGGACG---ACCGCGa-GUGGCGuGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 9940 | 0.72 | 0.089344 |
Target: 5'- uCGUCGCCgacCUGGCGCUCGuCCagccccaGCGCGg -3' miRNA: 3'- -GCGGCGGac-GACCGCGAGU-GG-------CGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 3897 | 0.72 | 0.087115 |
Target: 5'- aGCCaCCUGCUGGgucaGCUCGCCcagGCGCu -3' miRNA: 3'- gCGGcGGACGACCg---CGAGUGG---CGUGc -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 15817 | 0.74 | 0.062043 |
Target: 5'- cCGCCGCCgGCucuUGGCGCgggcgUACCgGCGCGc -3' miRNA: 3'- -GCGGCGGaCG---ACCGCGa----GUGG-CGUGC- -5' |
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359 | 3' | -62.9 | AC_000011.1 | + | 15950 | 0.74 | 0.060301 |
Target: 5'- uGCCGgC-GCUGGCGCcugaaGCCGCGCGu -3' miRNA: 3'- gCGGCgGaCGACCGCGag---UGGCGUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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