Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 359 | 5' | -55.5 | AC_000011.1 | + | 22400 | 0.66 | 0.594666 |
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Target: 5'- cGUCGUUGuUGGccagcugcaccacgcUGCGCCCCCAG--CGg -3' miRNA: 3'- -CAGCAAC-GCC---------------AUGUGGGGGUCuaGCa -5' |
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| 359 | 5' | -55.5 | AC_000011.1 | + | 10668 | 0.66 | 0.556617 |
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Target: 5'- -gCGUUGCGGUGug-CCCCGGuUCGa -3' miRNA: 3'- caGCAACGCCAUgugGGGGUCuAGCa -5' |
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| 359 | 5' | -55.5 | AC_000011.1 | + | 13690 | 0.67 | 0.512816 |
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Target: 5'- -gCGUUuacggGCGGcGCugCCCCGGcUCGUg -3' miRNA: 3'- caGCAA-----CGCCaUGugGGGGUCuAGCA- -5' |
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| 359 | 5' | -55.5 | AC_000011.1 | + | 18145 | 0.69 | 0.430027 |
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Target: 5'- -gCGg-GCGGU--GCCCCCGGGUCGc -3' miRNA: 3'- caGCaaCGCCAugUGGGGGUCUAGCa -5' |
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| 359 | 5' | -55.5 | AC_000011.1 | + | 10100 | 0.69 | 0.420233 |
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Target: 5'- aGUCGUUGCaGGUGCGCa-CCAGGUa-- -3' miRNA: 3'- -CAGCAACG-CCAUGUGggGGUCUAgca -5' |
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| 359 | 5' | -55.5 | AC_000011.1 | + | 11789 | 1.06 | 0.000876 |
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Target: 5'- uGUCGUUGCGGUACACCCCCAGAUCGUc -3' miRNA: 3'- -CAGCAACGCCAUGUGGGGGUCUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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