Results 1 - 20 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3590 | 3' | -41.9 | NC_001650.1 | + | 110482 | 0.67 | 1 |
Target: 5'- gGUGGAGCCGGCCAGgcaagAGg------- -3' miRNA: 3'- -CAUUUUGGUCGGUCaa---UCacaaauuc -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 183496 | 0.69 | 1 |
Target: 5'- uGUGG--UCAGCCAuGUUGGUGggUGGGc -3' miRNA: 3'- -CAUUuuGGUCGGU-CAAUCACaaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 16789 | 0.69 | 1 |
Target: 5'- uGUGG--UCAGCCAuGUUGGUGggUGGGc -3' miRNA: 3'- -CAUUuuGGUCGGU-CAAUCACaaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 180368 | 0.68 | 1 |
Target: 5'- -----cCCGGCCAGUgggaugcaugGGUGUcUGAGg -3' miRNA: 3'- cauuuuGGUCGGUCAa---------UCACAaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 13661 | 0.68 | 1 |
Target: 5'- -----cCCGGCCAGUgggaugcaugGGUGUcUGAGg -3' miRNA: 3'- cauuuuGGUCGGUCAa---------UCACAaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 72134 | 0.67 | 1 |
Target: 5'- -aAAGGCCAGCCGcccGGUGUUg--- -3' miRNA: 3'- caUUUUGGUCGGUcaaUCACAAauuc -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 121718 | 0.67 | 1 |
Target: 5'- ---uGACCAGCCGGUgggagaugcUGGUGg----- -3' miRNA: 3'- cauuUUGGUCGGUCA---------AUCACaaauuc -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 86195 | 0.66 | 1 |
Target: 5'- -gAGGGCCAGCCcaucaacgucAGggAGgaGUUUGAGa -3' miRNA: 3'- caUUUUGGUCGG----------UCaaUCa-CAAAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 96677 | 0.66 | 1 |
Target: 5'- ---cGACC-GUCAGUaUAGUGUUUAAu -3' miRNA: 3'- cauuUUGGuCGGUCA-AUCACAAAUUc -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 142228 | 0.67 | 1 |
Target: 5'- -cAAGAUauGCCAGUUAGUGUa---- -3' miRNA: 3'- caUUUUGguCGGUCAAUCACAaauuc -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 178188 | 0.67 | 1 |
Target: 5'- -gGGAGCCu-CCGGcucgAGUGUUUAAGg -3' miRNA: 3'- caUUUUGGucGGUCaa--UCACAAAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 11481 | 0.67 | 1 |
Target: 5'- -gGGAGCCu-CCGGcucgAGUGUUUAAGg -3' miRNA: 3'- caUUUUGGucGGUCaa--UCACAAAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 145714 | 0.67 | 1 |
Target: 5'- cGUAGAACUGGCaCAGgaGGUGgc-AGGg -3' miRNA: 3'- -CAUUUUGGUCG-GUCaaUCACaaaUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 182284 | 0.68 | 1 |
Target: 5'- ----cGCuCGGCUGGUUGGUGggUGGGu -3' miRNA: 3'- cauuuUG-GUCGGUCAAUCACaaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 15577 | 0.68 | 1 |
Target: 5'- ----cGCuCGGCUGGUUGGUGggUGGGu -3' miRNA: 3'- cauuuUG-GUCGGUCAAUCACaaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 44307 | 0.66 | 1 |
Target: 5'- -aGAGACUcucuaAGCUAGUUAGUGa-UAAGu -3' miRNA: 3'- caUUUUGG-----UCGGUCAAUCACaaAUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 178918 | 0.69 | 0.999999 |
Target: 5'- aGUAAAGCCAGCUGGUUA-UGcugaUAAGa -3' miRNA: 3'- -CAUUUUGGUCGGUCAAUcACaa--AUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 12211 | 0.69 | 0.999999 |
Target: 5'- aGUAAAGCCAGCUGGUUA-UGcugaUAAGa -3' miRNA: 3'- -CAUUUUGGUCGGUCAAUcACaa--AUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 1641 | 0.71 | 0.999982 |
Target: 5'- -gGGGGCCGGCCuugUGGUGUgaggUAAGc -3' miRNA: 3'- caUUUUGGUCGGucaAUCACAa---AUUC- -5' |
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3590 | 3' | -41.9 | NC_001650.1 | + | 168348 | 0.71 | 0.999982 |
Target: 5'- -gGGGGCCGGCCuugUGGUGUgaggUAAGc -3' miRNA: 3'- caUUUUGGUCGGucaAUCACAa---AUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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