miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
3590 5' -49.8 NC_001650.1 + 71048 0.68 0.983401
Target:  5'- cUCUggccCAGCAUGGCCAGCa-GCUUg -3'
miRNA:   3'- aAGAaua-GUCGUAUUGGUCGacCGAA- -5'
3590 5' -49.8 NC_001650.1 + 117701 0.68 0.981273
Target:  5'- ----gGUCAGggccaGUAACCAGCUGGUg- -3'
miRNA:   3'- aagaaUAGUCg----UAUUGGUCGACCGaa -5'
3590 5' -49.8 NC_001650.1 + 145620 0.7 0.952167
Target:  5'- ----gGUCAGCAggGAgUAGCUGGCUa -3'
miRNA:   3'- aagaaUAGUCGUa-UUgGUCGACCGAa -5'
3590 5' -49.8 NC_001650.1 + 76696 0.71 0.914484
Target:  5'- -cCUUGUCGGCGUggugcaggaacuuGACCGGCagggGGCUg -3'
miRNA:   3'- aaGAAUAGUCGUA-------------UUGGUCGa---CCGAa -5'
3590 5' -49.8 NC_001650.1 + 179132 1.03 0.021751
Target:  5'- gUUCUUAUCAGCAUAACCAGCUGGCUUu -3'
miRNA:   3'- -AAGAAUAGUCGUAUUGGUCGACCGAA- -5'
3590 5' -49.8 NC_001650.1 + 12425 1.03 0.021751
Target:  5'- gUUCUUAUCAGCAUAACCAGCUGGCUUu -3'
miRNA:   3'- -AAGAAUAGUCGUAUUGGUCGACCGAA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.