Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 3591 | 5' | -54 | NC_001650.1 | + | 152733 | 0.75 | 0.592298 |
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Target: 5'- aCUCUGAaaaGAgGGGACCUGGCAGg-CCa -3' miRNA: 3'- -GAGACUg--UUgUCUUGGGCCGUCgaGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 158915 | 0.75 | 0.571988 |
|
Target: 5'- uUCgUGGCGAUAGGggauGCCCGGCAGCagcugggugacaUCCg -3' miRNA: 3'- gAG-ACUGUUGUCU----UGGGCCGUCG------------AGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 138050 | 0.75 | 0.571988 |
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Target: 5'- -aCUGGaucaucucaGACAGGGCCUGGCAGC-CCa -3' miRNA: 3'- gaGACUg--------UUGUCUUGGGCCGUCGaGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 25131 | 0.76 | 0.551848 |
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Target: 5'- gCUCccggGACGGCAGAcACCUGGUccuGCUCCa -3' miRNA: 3'- -GAGa---CUGUUGUCU-UGGGCCGu--CGAGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 180754 | 1.11 | 0.003919 |
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Target: 5'- gCUCUGACAACAGAACCCGGCAGCUCCc -3' miRNA: 3'- -GAGACUGUUGUCUUGGGCCGUCGAGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 14047 | 1.11 | 0.003919 |
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Target: 5'- gCUCUGACAACAGAACCCGGCAGCUCCc -3' miRNA: 3'- -GAGACUGUUGUCUUGGGCCGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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