Results 81 - 86 of 86 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3591 | 5' | -54 | NC_001650.1 | + | 14644 | 0.68 | 0.935029 |
Target: 5'- uUCUcACAGUGGAACCCGGgCGGCcggCCu -3' miRNA: 3'- gAGAcUGUUGUCUUGGGCC-GUCGa--GG- -5' |
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3591 | 5' | -54 | NC_001650.1 | + | 14047 | 1.11 | 0.003919 |
Target: 5'- gCUCUGACAACAGAACCCGGCAGCUCCc -3' miRNA: 3'- -GAGACUGUUGUCUUGGGCCGUCGAGG- -5' |
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3591 | 5' | -54 | NC_001650.1 | + | 13844 | 0.67 | 0.960486 |
Target: 5'- gUCUGGCAGCGu--CCCccucagcaGGCAGCgCCu -3' miRNA: 3'- gAGACUGUUGUcuuGGG--------CCGUCGaGG- -5' |
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3591 | 5' | -54 | NC_001650.1 | + | 4218 | 0.68 | 0.929963 |
Target: 5'- uUCUGGCcaAugGGGACUCuguccgugGGCgAGCUCCg -3' miRNA: 3'- gAGACUG--UugUCUUGGG--------CCG-UCGAGG- -5' |
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3591 | 5' | -54 | NC_001650.1 | + | 3126 | 0.72 | 0.734228 |
Target: 5'- -cCUGGCAACAGAugacGCagaCGGCcacccGGCUCCc -3' miRNA: 3'- gaGACUGUUGUCU----UGg--GCCG-----UCGAGG- -5' |
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3591 | 5' | -54 | NC_001650.1 | + | 3045 | 0.71 | 0.826354 |
Target: 5'- -cCUGGCAACAGcugacgucgccgGGCCCGGaGGcCUCCc -3' miRNA: 3'- gaGACUGUUGUC------------UUGGGCCgUC-GAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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