Results 81 - 86 of 86 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3591 | 5' | -54 | NC_001650.1 | + | 134499 | 0.68 | 0.918542 |
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Target: 5'- gUCUGGCccuGgGGGGCCUGGCGGgcggggaaucgucCUCCu -3' miRNA: 3'- gAGACUGu--UgUCUUGGGCCGUC-------------GAGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 135987 | 0.69 | 0.907302 |
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Target: 5'- -cCUGGCcuuuCAGAGCauGGCGGcCUCCa -3' miRNA: 3'- gaGACUGuu--GUCUUGggCCGUC-GAGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 34684 | 0.69 | 0.901043 |
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Target: 5'- uUCUGACAcCGGGugcgcGCCCGGCuccugGGCcgCCc -3' miRNA: 3'- gAGACUGUuGUCU-----UGGGCCG-----UCGa-GG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 69068 | 0.69 | 0.894552 |
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Target: 5'- gUCUGGCGGCcauGGGCCCGggcgcGCGGCacgUCCc -3' miRNA: 3'- gAGACUGUUGu--CUUGGGC-----CGUCG---AGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 78819 | 0.69 | 0.894552 |
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Target: 5'- gUCUGccGCAGCGGcGCCCGcaCAGcCUCCa -3' miRNA: 3'- gAGAC--UGUUGUCuUGGGCc-GUC-GAGG- -5' |
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| 3591 | 5' | -54 | NC_001650.1 | + | 180754 | 1.11 | 0.003919 |
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Target: 5'- gCUCUGACAACAGAACCCGGCAGCUCCc -3' miRNA: 3'- -GAGACUGUUGUCUUGGGCCGUCGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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