Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3592 | 3' | -53 | NC_001650.1 | + | 117873 | 0.66 | 0.984131 |
Target: 5'- aGGuGGAGGUgaacucguGGGgcuGCUGCACCAc--- -3' miRNA: 3'- -CC-CCUCCA--------CCCac-UGAUGUGGUuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 70791 | 0.66 | 0.982391 |
Target: 5'- gGGGGAGGUGGuG-GACgggguacuggagacCACCGAc-- -3' miRNA: 3'- -CCCCUCCACC-CaCUGau------------GUGGUUauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 155621 | 0.66 | 0.982189 |
Target: 5'- gGGGGAggagaGGUGGGgaggGGCcuCACCGAc-- -3' miRNA: 3'- -CCCCU-----CCACCCa---CUGauGUGGUUauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 6888 | 0.66 | 0.982189 |
Target: 5'- gGGGGccgAGG-GGGU-ACUACcaugGCCAAUGAa -3' miRNA: 3'- -CCCC---UCCaCCCAcUGAUG----UGGUUAUU- -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 173595 | 0.66 | 0.982189 |
Target: 5'- gGGGGccgAGG-GGGU-ACUACcaugGCCAAUGAa -3' miRNA: 3'- -CCCC---UCCaCCCAcUGAUG----UGGUUAUU- -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 124897 | 0.66 | 0.982189 |
Target: 5'- aGGGaGAGGgGGGaGACgcggGCACCGc--- -3' miRNA: 3'- -CCC-CUCCaCCCaCUGa---UGUGGUuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 34654 | 0.66 | 0.982189 |
Target: 5'- gGGGGuGGUGGGUaccagcGcCUACAgCGAg-- -3' miRNA: 3'- -CCCCuCCACCCA------CuGAUGUgGUUauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 106980 | 0.66 | 0.982189 |
Target: 5'- aGGGGAGGggggaauguggGGGUGugUACguguACUAc--- -3' miRNA: 3'- -CCCCUCCa----------CCCACugAUG----UGGUuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 91051 | 0.66 | 0.979176 |
Target: 5'- uGGGGGcGGUGGGguacuccuucuugGGC-ACGCCcGUAAa -3' miRNA: 3'- -CCCCU-CCACCCa------------CUGaUGUGGuUAUU- -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 56286 | 0.66 | 0.977776 |
Target: 5'- gGGGGGGGUGGuGccUGGCgcGCGCCc---- -3' miRNA: 3'- -CCCCUCCACC-C--ACUGa-UGUGGuuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 53087 | 0.66 | 0.972605 |
Target: 5'- cGGGGAcGG-GGGUGguuuuGCgACGCCAAa-- -3' miRNA: 3'- -CCCCU-CCaCCCAC-----UGaUGUGGUUauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 181767 | 0.66 | 0.972605 |
Target: 5'- cGGGGAGGcGGccGGCUGCACa----- -3' miRNA: 3'- -CCCCUCCaCCcaCUGAUGUGguuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 41614 | 0.66 | 0.972605 |
Target: 5'- cGGGGcccgguuGGUGGGUGugUGuguCCAAa-- -3' miRNA: 3'- -CCCCu------CCACCCACugAUgu-GGUUauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 27222 | 0.66 | 0.972605 |
Target: 5'- aGGGGGGcGGGcGACUGCAgCCu---- -3' miRNA: 3'- cCCCUCCaCCCaCUGAUGU-GGuuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 15060 | 0.66 | 0.972605 |
Target: 5'- cGGGGAGGcGGccGGCUGCACa----- -3' miRNA: 3'- -CCCCUCCaCCcaCUGAUGUGguuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 109121 | 0.67 | 0.966617 |
Target: 5'- gGGGGAGGUGGacgaGGCggcGCACCc---- -3' miRNA: 3'- -CCCCUCCACCca--CUGa--UGUGGuuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 126783 | 0.67 | 0.9633 |
Target: 5'- gGGGGAGGgaugGGGcGACgACACUc---- -3' miRNA: 3'- -CCCCUCCa---CCCaCUGaUGUGGuuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 82722 | 0.67 | 0.95976 |
Target: 5'- aGGGGcGGUGGaUGACgcggGCGCCu---- -3' miRNA: 3'- -CCCCuCCACCcACUGa---UGUGGuuauu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 8779 | 0.67 | 0.95976 |
Target: 5'- gGGGGAGcGUGGGgGugUGagggACCGAUu- -3' miRNA: 3'- -CCCCUC-CACCCaCugAUg---UGGUUAuu -5' |
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3592 | 3' | -53 | NC_001650.1 | + | 175485 | 0.67 | 0.95976 |
Target: 5'- gGGGGAGcGUGGGgGugUGagggACCGAUu- -3' miRNA: 3'- -CCCCUC-CACCCaCugAUg---UGGUUAuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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