Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3594 | 5' | -50.7 | NC_001650.1 | + | 87110 | 0.66 | 0.993918 |
Target: 5'- gGUGUUGGGGGGgA--UGCCCugCu-- -3' miRNA: 3'- -CACAAUCUCUCgUuaACGGGugGcau -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 130092 | 0.66 | 0.992961 |
Target: 5'- -gGggGGAGAGCc---GCCCGCCa-- -3' miRNA: 3'- caCaaUCUCUCGuuaaCGGGUGGcau -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 108818 | 0.67 | 0.986239 |
Target: 5'- aUGUcAGAGAGCAg--GCCCugCa-- -3' miRNA: 3'- cACAaUCUCUCGUuaaCGGGugGcau -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 25672 | 0.68 | 0.982471 |
Target: 5'- -gGUcUGGAGAGCAGcgGCcCCACCa-- -3' miRNA: 3'- caCA-AUCUCUCGUUaaCG-GGUGGcau -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 58648 | 0.68 | 0.975429 |
Target: 5'- -gGUUGGGGGGCAGguggugGUCCGCCc-- -3' miRNA: 3'- caCAAUCUCUCGUUaa----CGGGUGGcau -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 155174 | 0.69 | 0.966517 |
Target: 5'- cGUGguagUGGAGGGCGAUcaUGCCCAgggCGUc -3' miRNA: 3'- -CACa---AUCUCUCGUUA--ACGGGUg--GCAu -5' |
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3594 | 5' | -50.7 | NC_001650.1 | + | 18910 | 1.07 | 0.012724 |
Target: 5'- gGUGUUAGAGAGCAAUUGCCCACCGUAc -3' miRNA: 3'- -CACAAUCUCUCGUUAACGGGUGGCAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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