miRNA: 3'- uaGAGGUauUUCCGGAGCCUCc----GCUG- -5'
miRNA: 3'- -UAGAGGUauUUCCGGAGCCUCcg-CUG- -5'
miRNA: 3'- -UAGAGgUauuUCCGGAGCCUCCGCUG- -5'
miRNA: 3'- uaGAGGUAU------UUCCGGAGCC---UCCGCUG- -5'
miRNA: 3'- -UAGAGGUAUUUCCGGAGCCUCCgCUG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3597 | 3' | -55.3 | NC_001650.1 | + | 81579 | 0.74 | 0.53809 |
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Target: 5'- -aCUCCAccAGGGCCUCGGGGucgccCGACg -3' miRNA: 3'- uaGAGGUauUUCCGGAGCCUCc----GCUG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 22279 | 0.76 | 0.451387 |
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Target: 5'- gAUCUCCAccGGGGCCUCGGAGcugGACc -3' miRNA: 3'- -UAGAGGUauUUCCGGAGCCUCcg-CUG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 110962 | 0.79 | 0.311346 |
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Target: 5'- uGUCUCgG---GGGCUUCGGGGGCGGCu -3' miRNA: 3'- -UAGAGgUauuUCCGGAGCCUCCGCUG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 71620 | 0.8 | 0.257824 |
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Target: 5'- -cCUCCAUGcgcucgGGGGCCUCGGgaaAGGCGGCc -3' miRNA: 3'- uaGAGGUAU------UUCCGGAGCC---UCCGCUG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 21776 | 0.95 | 0.02958 |
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Target: 5'- uAUCUCCAUAAAGGCCUCGGAGG-GACc -3' miRNA: 3'- -UAGAGGUAUUUCCGGAGCCUCCgCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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