miRNA: 3'- -UAGAGGUauuUCcGGAGCCUCCgCUG- -5'
miRNA: 3'- -UAGa--GG-UAUUUCCGGaGCCUCCGCUg -5'
miRNA: 3'- -UAGA--GG-UAUUUCCGGaGCC-UCCGCUg -5'
miRNA: 3'- -UAGa-GGUAUUUCCGG---AgCCUCCGCuG- -5'
miRNA: 3'- uagaGGUa--UUUCCGGA-GCCU---CCGCUG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 3597 | 3' | -55.3 | NC_001650.1 | + | 167562 | 0.69 | 0.832848 |
|
Target: 5'- cGUCUCCGggcAGaCCUCGGGGGaGACc -3' miRNA: 3'- -UAGAGGUauuUCcGGAGCCUCCgCUG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 167997 | 0.68 | 0.857095 |
|
Target: 5'- cAUCaagCCaAUGGGGGCC-CGGGGGUGGg -3' miRNA: 3'- -UAGa--GG-UAUUUCCGGaGCCUCCGCUg -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 168182 | 0.66 | 0.943816 |
|
Target: 5'- cAUCUagCCaAUGGGGGCC-CGGgGGGCGGg -3' miRNA: 3'- -UAGA--GG-UAUUUCCGGaGCC-UCCGCUg -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 168470 | 0.66 | 0.943816 |
|
Target: 5'- cAUUggCCAUGGAGGCCcugUgGGAGGUGcCc -3' miRNA: 3'- -UAGa-GGUAUUUCCGG---AgCCUCCGCuG- -5' |
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| 3597 | 3' | -55.3 | NC_001650.1 | + | 169980 | 0.68 | 0.841125 |
|
Target: 5'- ---gCCAaugGGAGGCCUcCGGGcccGGCGACg -3' miRNA: 3'- uagaGGUa--UUUCCGGA-GCCU---CCGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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