Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3603 | 3' | -53.3 | NC_001650.1 | + | 21811 | 0.7 | 0.848517 |
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Target: 5'- gCGCCCACCCUGUgcccgGGCAacaccguccuCAGUAUc--- -3' miRNA: 3'- -GCGGGUGGGACA-----UCGU----------GUCAUAcaug -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 52320 | 0.71 | 0.835346 |
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Target: 5'- cCGgCgGCCCUGUAuacuggacuugauguGUACAGUGUGUAa -3' miRNA: 3'- -GCgGgUGGGACAU---------------CGUGUCAUACAUg -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 131666 | 0.71 | 0.805779 |
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Target: 5'- gCGCCCccuCCCUGaggGGgGCGGUGgagGUGCu -3' miRNA: 3'- -GCGGGu--GGGACa--UCgUGUCAUa--CAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 123256 | 0.72 | 0.796711 |
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Target: 5'- -cUCCACCuCUGUGGCACGGUaaaaggggGUGUGg -3' miRNA: 3'- gcGGGUGG-GACAUCGUGUCA--------UACAUg -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 73012 | 0.72 | 0.749261 |
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Target: 5'- aCGCCCcucuCCCUG-AGCACcaugGUGUGCa -3' miRNA: 3'- -GCGGGu---GGGACaUCGUGuca-UACAUG- -5' |
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| 3603 | 3' | -53.3 | NC_001650.1 | + | 24455 | 1.1 | 0.004598 |
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Target: 5'- aCGCCCACCCUGUAGCACAGUAUGUACu -3' miRNA: 3'- -GCGGGUGGGACAUCGUGUCAUACAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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