Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 362 | 3' | -58.4 | AC_000011.1 | + | 13663 | 0.66 | 0.416938 |
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Target: 5'- cGUGGCCCCCUgCgacGCc-CGGGGAUc- -3' miRNA: 3'- -CACCGGGGGAgGa--CGuaGUCCUUGuc -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 13001 | 0.67 | 0.361751 |
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Target: 5'- -gGGCCUgUUCCUGaugCAGGAGgGGg -3' miRNA: 3'- caCCGGGgGAGGACguaGUCCUUgUC- -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 25951 | 0.67 | 0.344519 |
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Target: 5'- uGUuGCCCCCgcaggCCUGCAagCAGGAgACGa -3' miRNA: 3'- -CAcCGGGGGa----GGACGUa-GUCCU-UGUc -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 21189 | 0.67 | 0.344519 |
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Target: 5'- aUGGCCCUgagCUCCUGC-UCGccGGAGCc- -3' miRNA: 3'- cACCGGGG---GAGGACGuAGU--CCUUGuc -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 17928 | 0.67 | 0.336127 |
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Target: 5'- -aGcGUCUCCUCC-GCAUCGGG-GCGGg -3' miRNA: 3'- caC-CGGGGGAGGaCGUAGUCCuUGUC- -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 11428 | 0.67 | 0.327885 |
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Target: 5'- aUGGCCUCCagcaggUCCcacaggUGCAUCAGGccCAGg -3' miRNA: 3'- cACCGGGGG------AGG------ACGUAGUCCuuGUC- -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 36002 | 0.68 | 0.288932 |
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Target: 5'- ---cUCCCCUCCUGCA-CAGGcAGCAa -3' miRNA: 3'- caccGGGGGAGGACGUaGUCC-UUGUc -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 4921 | 0.71 | 0.178514 |
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Target: 5'- gGUGGcCCCCCUCCUcCGggAGGAcgGCAGc -3' miRNA: 3'- -CACC-GGGGGAGGAcGUagUCCU--UGUC- -5' |
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| 362 | 3' | -58.4 | AC_000011.1 | + | 13067 | 1.09 | 0.00025 |
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Target: 5'- gGUGGCCCCCUCCUGCAUCAGGAACAGg -3' miRNA: 3'- -CACCGGGGGAGGACGUAGUCCUUGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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