Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3621 | 3' | -54.1 | NC_001650.1 | + | 61946 | 0.66 | 0.952525 |
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Target: 5'- aGGAgucAGG-GACUGUgUCaGCUGUCUg -3' miRNA: 3'- aCCU---UCCaCUGGCAgAGcCGAUAGAa -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 46604 | 0.66 | 0.952525 |
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Target: 5'- cGGAGGG-GGCCGcCgCGGCgcAUCUg -3' miRNA: 3'- aCCUUCCaCUGGCaGaGCCGa-UAGAa -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 117979 | 0.67 | 0.943916 |
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Target: 5'- gGGGAGGgcGCCGaC-CGGCUGUCg- -3' miRNA: 3'- aCCUUCCacUGGCaGaGCCGAUAGaa -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 114908 | 0.67 | 0.934347 |
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Target: 5'- aGGGAGGUGGCCGgguugccCUccacCGGCUugagguggacgGUCUUg -3' miRNA: 3'- aCCUUCCACUGGCa------GA----GCCGA-----------UAGAA- -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 91035 | 0.77 | 0.468026 |
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Target: 5'- aGGggGGUGACCGUCUUGGg------ -3' miRNA: 3'- aCCuuCCACUGGCAGAGCCgauagaa -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 107022 | 0.79 | 0.354624 |
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Target: 5'- aUGGAgcGGGUGGCCGUCcCGGC-GUCUUa -3' miRNA: 3'- -ACCU--UCCACUGGCAGaGCCGaUAGAA- -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 123856 | 0.8 | 0.302103 |
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Target: 5'- cUGGGAGGUGAucacggucCCGgggaUCUCGGCUAUCUg -3' miRNA: 3'- -ACCUUCCACU--------GGC----AGAGCCGAUAGAa -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 69118 | 0.82 | 0.262027 |
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Target: 5'- gGGAAGGcGACCagguUCUCGGCUAUCUUg -3' miRNA: 3'- aCCUUCCaCUGGc---AGAGCCGAUAGAA- -5' |
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| 3621 | 3' | -54.1 | NC_001650.1 | + | 37356 | 1.06 | 0.0074 |
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Target: 5'- cUGGAAGGUGACCGUCUCGGCUAUCUUg -3' miRNA: 3'- -ACCUUCCACUGGCAGAGCCGAUAGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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