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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3623 | 3' | -49.4 | NC_001650.1 | + | 73520 | 0.66 | 0.999107 |
Target: 5'- cGCC-CAUGUCCGUGgUGAUgGAGg-- -3' miRNA: 3'- -UGGuGUGUAGGCAUgACUAaCUCaca -5' |
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3623 | 3' | -49.4 | NC_001650.1 | + | 143016 | 0.66 | 0.998399 |
Target: 5'- gACCGCcucCAUCugCGUGCUGugcGAGUGUa -3' miRNA: 3'- -UGGUGu--GUAG--GCAUGACuaaCUCACA- -5' |
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3623 | 3' | -49.4 | NC_001650.1 | + | 51268 | 0.68 | 0.994794 |
Target: 5'- gACCGCAgGUUCGgGCUGAUgggcugcugcGAGUGg -3' miRNA: 3'- -UGGUGUgUAGGCaUGACUAa---------CUCACa -5' |
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3623 | 3' | -49.4 | NC_001650.1 | + | 38098 | 0.7 | 0.982633 |
Target: 5'- uACCACACAcCCGUAUaGAUUGuGUu- -3' miRNA: 3'- -UGGUGUGUaGGCAUGaCUAACuCAca -5' |
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3623 | 3' | -49.4 | NC_001650.1 | + | 38343 | 0.96 | 0.090158 |
Target: 5'- cACCACACAUCCGUACUGAUUaGGUGUa -3' miRNA: 3'- -UGGUGUGUAGGCAUGACUAAcUCACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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