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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3623 | 5' | -47.9 | NC_001650.1 | + | 19437 | 0.66 | 0.999634 |
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Target: 5'- aGAGCugAGUagaccgagggccugCUGUugGGGUGagcUGUGGa -3' miRNA: 3'- -UUUGugUUA--------------GAUAugCCCAC---ACACCa -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 49108 | 0.67 | 0.998756 |
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Target: 5'- uGGAC-CAGUCUAgaUACGuGGUG-GUGGa -3' miRNA: 3'- -UUUGuGUUAGAU--AUGC-CCACaCACCa -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 47617 | 0.68 | 0.997808 |
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Target: 5'- uGAgGC-GUCUGUgGCGGGggcgGUGUGGUu -3' miRNA: 3'- uUUgUGuUAGAUA-UGCCCa---CACACCA- -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 175241 | 0.69 | 0.993134 |
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Target: 5'- uGGugGgGG-CUAUGgGGGUGUGUGGg -3' miRNA: 3'- -UUugUgUUaGAUAUgCCCACACACCa -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 8534 | 0.69 | 0.993134 |
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Target: 5'- uGGugGgGG-CUAUGgGGGUGUGUGGg -3' miRNA: 3'- -UUugUgUUaGAUAUgCCCACACACCa -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 38132 | 0.7 | 0.990868 |
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Target: 5'- -uACACcuAAUCaGUACGGaUGUGUGGUg -3' miRNA: 3'- uuUGUG--UUAGaUAUGCCcACACACCA- -5' |
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| 3623 | 5' | -47.9 | NC_001650.1 | + | 38309 | 1.06 | 0.027385 |
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Target: 5'- uAAACACAAUCUAUACGGGUGUGUGGUa -3' miRNA: 3'- -UUUGUGUUAGAUAUGCCCACACACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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