Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 3624 | 5' | -45.6 | NC_001650.1 | + | 164928 | 0.66 | 0.999987 |
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Target: 5'- cCUCGCUCAGgcgGCCcuggAGAGCUucuGUCuguGCAg -3' miRNA: 3'- -GAGUGAGUCaa-CGG----UUUUGA---UAG---UGU- -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 149355 | 0.67 | 0.999982 |
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Target: 5'- uCUCAC-CAGUuugucuuuagcUGCCAuuuugaaaGAGCUAauUCACAg -3' miRNA: 3'- -GAGUGaGUCA-----------ACGGU--------UUUGAU--AGUGU- -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 149147 | 0.67 | 0.999967 |
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Target: 5'- aUCuGCUCGGUUGCCcggGAGACgggcuccagUGUCACc -3' miRNA: 3'- gAG-UGAGUCAACGG---UUUUG---------AUAGUGu -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 149431 | 0.67 | 0.999955 |
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Target: 5'- gCUUAUUCuuaguGUUGCuUGAGACUGUUACAu -3' miRNA: 3'- -GAGUGAGu----CAACG-GUUUUGAUAGUGU- -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 92517 | 0.7 | 0.999077 |
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Target: 5'- gCUCAC-CAGcgUGCuCGAGACcGUCACGu -3' miRNA: 3'- -GAGUGaGUCa-ACG-GUUUUGaUAGUGU- -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 38410 | 0.7 | 0.998866 |
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Target: 5'- -aCAUUCAG-UGCUuAAACUGUCGCc -3' miRNA: 3'- gaGUGAGUCaACGGuUUUGAUAGUGu -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 46332 | 0.71 | 0.998318 |
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Target: 5'- gCUCGCUCcccauugGCCGcucGAGCUGUCACu -3' miRNA: 3'- -GAGUGAGucaa---CGGU---UUUGAUAGUGu -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 62730 | 0.71 | 0.996545 |
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Target: 5'- -cCACggGGUUGCCAuAGCUGUCuACGg -3' miRNA: 3'- gaGUGagUCAACGGUuUUGAUAG-UGU- -5' |
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| 3624 | 5' | -45.6 | NC_001650.1 | + | 38584 | 1.1 | 0.02813 |
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Target: 5'- cCUCACUCAGUUGCCAAAACUAUCACAc -3' miRNA: 3'- -GAGUGAGUCAACGGUUUUGAUAGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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